Izvorni znanstveni članak
https://doi.org/10.18054/pb.v124i3-4.24754
Status of the BOLD reference library of DNA barcodes of caddisflies (Insecta: Trichoptera) from the Western Balkans
Lejla Ušanović
; University of Sarajevo – Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina
Dalila Destanović
orcid.org/0000-0002-3744-5789
; University of Vienna, Faculty of Life Sciences, Vienna, Austria
Lejla Lasić
; University of Sarajevo – Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina
Jasna Hanjalić Kurtović
orcid.org/0000-0002-8693-7886
; University of Sarajevo – Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina
Filipe O. Costa
orcid.org/0000-0001-5398-3942
; Centre of Molecular and Environmental Biology (CBMA), University of Minho, Braga, Portugal; Institute of Science and Innovation for Bio-Sustainability (IB-S), University of Minho, Braga, Portugal
Belma Kalamujić Stroil
orcid.org/0000-0002-4539-1266
; University of Sarajevo – Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina
Prilozi: Supplementary 1.pdf Supplementary 2.pdf Supplementary 3.pdf Supplementary 4.pdf Supplementary 5.pdf Supplementary 6.pdf Supplementary 7.pdf Supplementary 8.pdf
Sažetak
Background and purpose: Available data in research literature suggest that the Western Balkan countries hold a rich diversity of caddisflies. Assessment and biomonitoring of such rich diversity could be facilitated through DNA-based high-throughput approaches like DNA metabarcoding that depend on the availability of comprehensive reference libraries.
Materials and methods: We assessed the status of the COI barcode sequence data for a total of 112 caddisflies species in the investigated region by determining the gaps in representative sequences in the Barcode of Life Data System (BOLD) and examining the accuracy of available records using the Barcode, Audit and Grade System (BAGS).
Results: Results revealed a considerable underrepresentation of surveyed geographic region in BOLD records for the target insect group. Moreover, the large majority of the species records were rated “discordant” (72.80% grade E), and only 15.20% were classified as “consolidated concordance or basal concordance” (3.20% grade A and 12.00% B). Approximately 3.20% of the records pertaining to species occurring in multiple BINs (Barcode Index Number) and 8.80% were poorly represented (i.e., less than three specimens, grade D). A fraction of the species graded discordant were deemed concordant after detailed inspection of individual data, decreasing by 14.07%.
Conclusions: The assessment of the current state of BOLD entries indicated that DNA barcoding is still not widely applied in Albania, Bosnia and Herzegovina, Montenegro, North Macedonia, Serbia, and Slovenia, emphasizing that Croatia has the most barcoded caddisflies species. The finding that available BOLD Trichopteran records for investigated countries were mainly graded as “discordant” indicates the need for better quality control of reference libraries.
Ključne riječi
COI gene; BOLD; BAGS; diversity assessment; biomonitoring; aquatic insects
Hrčak ID:
301214
URI
Datum izdavanja:
22.9.2022.
Posjeta: 237 *