Izvorni znanstveni članak
https://doi.org/10.2478/aiht-2023-74-3727
The effects of ketamine on viability, primary DNA damage, and oxidative stress parameters in HepG2 and SH-SY5Y cells
Andreja Jurič
orcid.org/0000-0002-9279-5159
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Blanka Tariba Lovaković
orcid.org/0000-0001-5908-506X
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Antonio Zandona
orcid.org/0000-0002-3937-3520
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Dubravka Rašić
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Martin Češi
; University of Zagreb Faculty of Food Technology and Biotechnology, Zagreb, Croatia
Alica Pizent
orcid.org/0000-0003-0216-0166
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Marijana Neuberg
; University North, University Centre Varaždin, Varaždin, Croatia
Irena Canjuga
; University North, University Centre Varaždin, Varaždin, Croatia
Maja Katalinić
orcid.org/0000-0001-7043-4291
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Ana Lucić Vrdoljak
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Arnes Rešić
; Children’s Hospital Zagreb Department of Paediatrics, Zagreb, Croatia
Irena Brčić Karačonji
orcid.org/0000-0002-1151-8839
; Institute for Medical Research and Occupational Health, Zagreb, Croatia, University of Rijeka Faculty of Health Studies, Rijeka, Croatia
Sažetak
Ketamine is a dissociative anaesthetic used to induce general anaesthesia in humans and laboratory animals. Due to its hallucinogenic and dissociative effects, it is also used as a recreational drug. Anaesthetic agents can cause toxic effects at the cellular level and affect cell survival, induce DNA damage, and cause oxidant/antioxidant imbalance. The aim of this study was to explore these possible adverse effects of ketamine on hepatocellular HepG2 and neuroblastoma SH-SY5Y cells after 24-hour exposure to a concentration range covering concentrations used in analgesia, drug abuse, and anaesthesia (0.39, 1.56, and 6.25 μmol/L, respectively). At these concentrations ketamine had relatively low toxic outcomes, as it lowered HepG2 and SH-SY5Y cell viability up to 30 %, and low, potentially repairable DNA damage. Interestingly, the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione (GSH) remained unchanged in both cell lines. On the other hand, oxidative stress markers [superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT)] pointed to ketamine-induced oxidant/antioxidant imbalance.
Ključne riječi
anaesthetic; antioxidant enzymes; drug abuse; ROS; toxicity
Hrčak ID:
304381
URI
Datum izdavanja:
20.6.2023.
Posjeta: 803 *