Izvorni znanstveni članak
https://doi.org/10.2478/aiht-2023-74-3706
Is alumina suitable for solid phase extraction of catecholamines from brain tissue?
Duško Mirković
; University of Belgrade Faculty of Pharmacy, Department of Medical Biochemistry, Belgrade, Serbia
Anđelo Beletić
orcid.org/0000-0001-8975-9600
; University of Zagreb Faculty of Veterinary Medicine, Internal Diseases Clinic, Laboratory for Proteomics, Zagreb, Croatia 3 University Clinical Centre of Serbia, Centre for Medical Biochemistry, Belgrade, Serbia
Miroslav Savić
; University of Belgrade Faculty of Pharmacy, Department of Pharmacology, Belgrade, Serbia
Neda Milinković
orcid.org/0000-0002-2641-9817
; University of Belgrade Faculty of Pharmacy, Department of Medical Biochemistry, Belgrade, Serbia
Marija Sarić Matutinović
; University of Belgrade Faculty of Pharmacy, Department of Medical Biochemistry, Belgrade, Serbia
Ivan Jančić
; University of Belgrade Faculty of Pharmacy, Department of Microbiology and Immunology, Belgrade, Serbia
Sažetak
Occupational and environmental toxicology specialists find catecholamine fluctuations in brain tissue relevant for research of neurotoxicity, such as that induced by manganese or zinc, pesticides, industrial solvents, plastic, air pollution, or irradiation. Considering that catecholamine tissue concentrations are generally very low, their extraction requires a reliable and optimal method that will achieve maximum recovery and minimise other interferences. This study aimed to evaluate whether the aluminium (III) oxide (Al2O3, alumina) based cartridges designed for catecholamine isolation from plasma could be used for solid-phase extraction (SPE) of catecholamine from the brain tissue. To do that, we homogenised Wistar rat brain tissue with perchloric acid and compared three extraction techniques: SPE, the routine filtration through a 0.22 μm membrane filter, and their combination. In the extracts, we compared relative chromatographic catecholamine mobility measured with high performance liquid chromatography with electrochemical detection. Chromatographic patterns for norepinephrine and epinephrine were similar regardless of the extraction technique, which indicates that the alumina cartridge is good enough to isolate them from brain tissue. However, the dopamine pattern was unsatisfactory, and further experiments are needed to identify the issue and optimise the protocol.
Ključne riječi
catecholamines; aluminium oxide; solid phase extraction; brain; tissue
Hrčak ID:
304384
URI
Datum izdavanja:
20.6.2023.
Posjeta: 267 *