Izvorni znanstveni članak
https://doi.org/10.5599/jese.1664
Self-assembling nanomaterial-based peptide surface for target cell adhesion
Hasret Turkmen
; Izmir Biomedicine and Genome Center, Balcova 35340, Izmir, Turkey; Department of Biochemistry, Ege University, Izmir, Turkey
Sažetak
Non-covalent modification of electrode surfaces with nanoparticle-based peptides does not change the chemical properties of the electrode but allows electrochemical measurement of cell adhesion. This study examines the effect of self-modified nanomaterial/peptide surfaces on cell adhesion. This adhesion to the surface is caused by the negative Gibs free energy formed in the system because of the presence of -0H, sulfur, carbonyl, or reactive groups. A cheaper and more practical method for electrode surfaces targeting cell adhesion, which does not use heavy chemicals and EDC/NHS chemistry, is used in this work. Thanks to the bioactive materials immobilized on the screen-printed carbon electrode (SPCE) surface in a controlled manner and the surface chemistry offered by these materials, a biocompatible self-assembling nanomaterial-based peptide surface platform is created, and cell adhesion is measured by an electrochemical technique. After the characterization steps, electrochemical techniques created a calibration curve of the current value as a function of concentration for each cell line. The adhesion of the generated bioactive electrode surfaces to the selected cell lines was examined comparatively.
Ključne riječi
Screen-printed carbon electrode (SPCE); gold nanoparticles; biofunctionalization; cell lines
Hrčak ID:
305149
URI
Datum izdavanja:
28.6.2023.
Posjeta: 160 *