Veterinarski arhiv, Vol. 93 No. 2, 2023.
Izvorni znanstveni članak
https://doi.org/10.24099/vet.arhiv.2146
Novi VP2/VP3 rekombinantni senekavirus A izoliran u sjevernoj Kini
Yingru Ma
; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China
Limei Zheng
; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China
Chen Li
; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Xiaoyan Wu
; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Jianli Shi
; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Chang Liu
; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Hong Han
; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Shaojian Xu
; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Nataliia Hrabchenko
; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Mengjie Huang
; College of Animal Science and Veterinary Medicine Shandong Agricultural University, Taian, China
Jianhua Qiu
; College of Animal Science and Veterinary Medicine Shandong Agricultural University, Taian, China
Jun Li
; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China; Shandong Provincial Key Laboratory of Animal Disease Control & Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Xianjie Han
; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China
Sažetak
Senekavirus A (SVA), prije nazivan virusom doline Seneca Valley, jedini je pripadnik roda senekavirusa u porodici
Picornaviridae. Virus je slučajno otkriven 2002. i nazvan virusom doline Seneca 001 (SVV-001). SVA je novi patogen
koji može uzrokovati vezikularne lezije i prolaznu epidemiju novorođene prasadi s naglim gubicima u proizvodnji. U
ovom je istraživanju soj SVA izoliran u populaciji svinja iz provincije Shandong u Kini i identificiran kao SVA-CHSDFX-2022. Kompletni genom izolata SVA imao je 7282 nukleotida (nt) u dužini i sadržavao je jedan otvoreni okvir
za očitavanje (ORF), bez poli-A repova. Filogenetska je analiza pokazala da izolat u velikoj mjeri sadržava genomsku
organizaciju i nukleotidne identitete, od 90,5 % do 99,6 %, s drugim poznatim SVA izolatima. Karakterizacija virusa
je pokazala da ima veliku sposobnost rasta in vitro. Pronađena je rekombinacija izolata SVA-CH-SDFX-između
nukleotida 1836 i 2710 što je uključilo regiju gena VP2 (parcijalno) i gena VP3 (parcijalno). Zbog visoke stope
infektivnosti i golemih ekonomskih gubitaka važan je brži razvoj cjepiva i bolje razumijevanje zaraze. Rezultati ovog
istraživanja pružaju korisne podatke za proučavanje SVA virusa, posebno s obzirom na njegovu epidemiologiju u
svinja i regionalnu prekograničnu kontrolu vezikularnih bolesti.
Ključne riječi
senekavirus A; svinje; izolacija virusa; IFA; evolucijska analiza; Western blotting
Hrčak ID:
305599
URI
Datum izdavanja:
25.6.2023.
Posjeta: 157 *