Original scientific paper

https://doi.org/10.46419/vs.55.2.10

Distribution of sialoglycoconjugates – gangliosides and PSA-NCAM in the brain of two venomous snakes: Vipera ammodytes and Vipera berus bosniensis

Barbara Viljetić ; Katedra za medicinsku kemiju, biokemiju i kliničku kemiju, Medicinski fakultet, Osijek, Hrvatska
Dario Dragun orcid.org/0009-0009-0814-6075 ; Katedra za medicinsku biologiju i genetiku, Medicinski fakultet, Osijek, Hrvatska
Irena Labak orcid.org/0000-0001-9394-5131 ; Odjel za biologiju, Sveučilište J. J. Strossmayer, Osijek, Hrvatska
Tomislav Bogdanović ; Odjel za biologiju, Sveučilište J. J. Strossmayer, Osijek, Hrvatska
Dušan Jelić ; BIOTA j.d.o.o., Grubišno polje, Hrvatska
Marija Heffer orcid.org/0000-0001-6770-7359 ; Katedra za medicinsku biologiju i genetiku, Medicinski fakultet, Osijek
Mislav Kovačić ; Odjel za biologiju, Sveučilište J. J. Strossmayer, Osijek, Hrvatska
Senka Blažetić orcid.org/0000-0003-1841-5063 ; Odjel za biologiju, Sveučilište J. J. Strossmayer, Osijek, Hrvatska

Abstract

The Bosnian adder (Vipera berus bosniensis) and the horned viper (Vipera ammodytes) are two venomous snake species with different ecological preferences. The Bosnian adder occurs in a range of habitats and is endemic to the Balkan Peninsula, while the horned viper thrives in dry, rocky areas with little vegetation. The horned viper is best known for its highly venomous venom, making it the most dangerous of the European vipers. The aim of this study was to compare the expression and distribution of complex gangliosides and to identify migratoryzones in the brain of Bosnian adder and horned viper. Immunohistochemistry was performed using specific antibodies for the major brain gangliosides (GM1, GD1a, GD1b, GT1b) and PSA NCAM and analysed in different
brain regions. Both snake species showed expression of all four complex gangliosides with similar distribution patterns. GD1b was the most prominent ganglioside expressed in all brain structures, while GM1 showed varying distribution between the species. The strongest expression of PSA NCAM was observed in the periventricular zones of the telencephalon, suggesting that these areas are associated with neurogenesis, whereas other regions with lower expression may serve as migratory zones. In addition, it is important to note that the specific distribution of gangliosides and PSA NCAM may be influenced by factors such as brain region, developmental stage, and species-specific characteristics.

Keywords

gangliosides; PSA-NCAM; brain; venomous snake

Hrčak ID:

305878

URI

https://hrcak.srce.hr/305878

Publication date:

13.7.2023.

Article data in other languages: croatian

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