Izvorni znanstveni članak
https://doi.org/10.2478/acph-2023-0037
The impact of cryoprotectant exposure time on post-thaw viability of autologous and allogeneic hematopoietic stem cells and leukocyte subpopulations
VLADIMIRA RIMAC
orcid.org/0000-0002-4598-4870
INES BOJANIĆ
orcid.org/0000-0002-2985-7847
; Department of Transfusion Medicine and Transplantation Biology, University Hospital Centre Zagreb, 10000 Zagreb, Croatia
*
SANJA DABELIĆ
; University of Zagreb Faculty of Pharmacy and Biochemistry, 10000 Zagreb, Croatia
BRANKA GOLUBIĆ ĆEPULIĆ
; University of Zagreb School of Medicine, 10000 Zagreb, Croatia
* Dopisni autor.
Sažetak
Although the use of cryoprotectant dimethyl sulfoxide (DMSO) is the gold standard in cryopreservation of hematopoietic stem cells, it is well known that it has a negative effect on cell viability. The aim of this prospective study was to examine how the length of post-thaw exposure to DMSO affects the cell viability and stability of peripheral blood stem cell (PBSC) samples. Additionally, the effects of donor type and pre-cryopreservation storage time on post-thaw viability during the stability study were evaluated. In 30 autologous and 30 allogeneic PBSC samples viable CD34+, CD14+, CD19+, CD16+/56+, and CD3+ cells were determined immediately after thawing, and one- and three-hours post-thaw.
Analysis of the absolute count of viable cells in thawed samples showed a significant difference between all measurement points for CD34+ (p < 0.001), CD14+ (p < 0.001), and CD19+ cells (p < 0.001). No significant differences were observed for post-thaw stability of allogeneic samples analysed between products stored before cryopreservation ≥ 24 hours (N = 20), and those stored < 24 hours (N = 10), except for viable CD3+/CD4+ cells after three hours post-thaw (p = 0.028). In conclusion, DMSO had different effects on leukocyte subpopulations in cryopreserved PBSC samples. The type of donors and the length of storage before cryopreservation did not affect the post-thaw stability of cryopreserved PBSC samples.
Ključne riječi
cryopreservation; sample stability; viability; peripheral blood stem cells
Hrčak ID:
307927
URI
Datum izdavanja:
30.12.2023.
Posjeta: 529 *