Izvorni znanstveni članak
https://doi.org/10.2478/acph-2023-0038
Preparation of astaxanthin/zeaxanthin-loaded nanostructured lipid carriers for enhanced bioavailability: Characterization-, stability- and permeability study
KRISTINA RADIĆ
ANA ISABEL BARBOSA
; LAQV, REQUIMTE, Departamento de Ciências Químicas, University of Porto, 4050-313 Porto, Portugal
SALETTE REIS
; LAQV, REQUIMTE, Departamento de Ciências Químicas, University of Porto, 4050-313 Porto, Portugal
MARIJAN MARIJAN
; Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
SOFIA ANTUNES COSTA LIMA
; LAQV, REQUIMTE, Instituto de Ciências Biomédicas de Abel Salazar, University of Porto, 4050-313 Porto, Portugal
DUBRAVKA VITALI ČEPO
; Department of Food Chemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
Sažetak
Astaxanthin (ASTA) and zeaxanthin (ZEA) are xanthophyll carotenoids showing a wide spectrum of health-promoting properties. However, their utilization is limited, mostly due to poor water solubility, limited bioavailability, and a tendency to oxidate, as well as photo- and thermal instability. The aim of this work was to develop ASTA- and ZEA-loaded nanostructured lipid carriers (NLCs) that would protect them against degradation and improve their intestinal stability/permeability. Obtained NLCs were characterized by an effective diameter of 294 nm for ASTA-NLC and 280 nm for ZEA-NLC; polydispersity index (PDI) lower than 0.2; and zeta potential of –29.4 mV and –29.0 mV, respectively. Interestingly, despite similar physicochemical characteristics, our investigation revealed differences in the encapsulation efficiency of ASTA-NLC and ZEA-NLC (58.0 % vs. 75.5 %, respectively). Obtained NLCs were stable during a 21 day-storage period in the dark at room temperature or at 4 °C. Investigation of gastrointestinal stability showed no change in effective diameter and PDI under gastric conditions while both parameters significantly changed under intestinal conditions. Our results showed for the first time that both ASTA- and ZEA-NLCs intestinal absorption investigated in the in vitro model is significantly increased (in relation to pure compounds) and is affected by the presence of mucus. This study provides useful data about the advantages of using NLC as a delivery system for ASTA and ZEA that might facilitate their applications in the food and pharmaceutical industry.
Ključne riječi
cryopreservation; sample stability; viability; peripheral blood stem cells
Hrčak ID:
307928
URI
Datum izdavanja:
30.12.2023.
Posjeta: 162 *