Original scientific paper
https://doi.org/10.15644/asc57/3/2
Assessment of Cytotoxic and Genotoxic Effect of Modern Dental Materials in vivo
Milena Trutina Gavran
orcid.org/0000-0001-5235-5215
; Department of Morphology and Anthropology, Study of Dental Medicine, School of Medicine, University of Mostar, Mostar, Bosnia and Herzegovina
Davor Željezić
; Division for Mutagenesis, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Lara Vranić
; School of Dental Medicine University of Zagreb, Zagreb, Croatia
Dubravka Negovetić Vranić
; Department of Paediatric Dentistry, School of Dental Medicine University of Zagreb, Zagreb, Croatia
Lana Grabarević
; Private dental clinic, Zagreb, Croatia
Danijela Jurić-Kaćunarić
orcid.org/0000-0003-2929-0905
; Private dental clinic, Gaggenau, Germany
Antonija Tadin
orcid.org/0000-0002-5365-9816
; Department of Restorative Dental Medicine and Endodontics, Study of Dental Medicine, School of Medicine, University of Split, Split, Croatia
Sanja Šegović
; Department of Endodontics and Restorative Dental Medicine, School of Dental Medicine University of Zagreb, Zagreb, Croatia
Nada Galić
; Department of Endodontics and Restorative Dental Medicine, School of Dental Medicine University of Zagreb, Zagreb, Croatia
Abstract
Objectives: The aim of the study was to assess the biocompatibility of modern composite and amalgam dental fillings. Material and Methods: The research was conducted on 150 healthy patients between the ages of 10 and 20 who had amalgam and composite fillings between 6 and 12 months.
Under in vivo conditions, a swab of buccal cells near the fillings was taken, and the cytotoxic and genotoxic impact of composite and amalgam fillings on these cells was analyzed using the extended micronucleus test (cytomeassay). Results: The results showed statistically significant differences between the groups of subjects with amalgam and composite fillings and subjects without fillings for the following parameters: number of micronuclei (p=0.006), number of buds (p<0.001), number of binuclear cells (p<0.001), number of nucleoplasmic bridges (p<0.001).The number of micronuclei was statistically significantly higher in the group of subjects with amalgam and composite fillings compared to the group without fillings. The results for nuclear buds, for the number of binuclear cells and the number of nucleoplasmic bridges showed that the group with amalgam fillings had a statistically significantly higher number of these changes compared to other groups.The results of the analysis of the relationship between the parameters of the micronucleus test and the number of amalgam and composite surfaces did not show statistically significant values. Parameters indicating cell cytotoxicity were not statistically significantly elevated in subjects with fillings.The results of the analysis of the influence of the patients’ lifestyle on the results of the micronucleus test showed statistically significant results for certain predictors (diagnostic X-ray radiation, coffee consumption, consumption of cooked, dried meat and baked food). Conclusion: Based on the results, it can be concluded that the buccal cells of subjects with amalgam fillings showed the highest degree of genotoxic changes, followed by those with composite fillings and the least buccal cells of patients without fillings.
Keywords
Materials Testing; Composite Resins; Dental Amalgam; Micronucleus Tests; Cytotoxins; cytotoxicity; genotoxicity; composite materials; buccal epithelial cells
Hrčak ID:
308159
URI
Publication date:
27.9.2023.
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