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Senile Cataract and the Absorption Activity of Cytochrome C Oxidase

Z. Balog
J. Šikić
B. Vojniković
S. Balog


Puni tekst: engleski pdf 53 Kb

str. 33-36

preuzimanja: 457

citiraj


Sažetak

The aim of the study was to determine the activity of cytochrome c oxidase in mitochondrial
fractions of cataractogenic epithelial cells of lenses of 60 patients (34 females
and 26 males). According to clinical criteria patients were divided into three groups:
group 1 – patients with senile cataract, group 2 – active smokers with senile cataract,
and group 3 – diabetic patients with cataract as diabetic complication. In the extracted
lenses we determined the absorption activity of mitochondrial enzyme cytochrome c oxidase.
Biochemical researches using the method of Yonetani and Ray13 were applied. We
also applied the statistical Student t-test (p < 0.05) and the variance analysis (R. Fisher)
with three parameters and Snedecor F distribution (s = p < 0.001). The activity of
cytochrome c oxidase in mitochondrial fraction of lens epithelial cells is twice as low in
patients who are active smokers than in the non-smoking patients with senile cataract.
We measured a significantly different activity of cytochrome c oxidase between active
smokers and the non-smoking patients with senile diabetic cataract (tp < 0.05, sp < 0.001).
Cigarette smoke decreases the activity of cytochrome c oxidase in mitochondrial fraction
of lens epithelial cells. This enzyme is a terminal oxidase in the synthesis of ATPs. We
suggest that smoking decreases the synthesis of energy in the lens of cigarette smokers.
This emphasizes the significance of giving up smoking in order to preserve the structure
and function of the lens.

Ključne riječi

Hrčak ID:

28469

URI

https://hrcak.srce.hr/28469

Posjeta: 781 *