Izvorni znanstveni članak
The Impact of Blue Light Irradiation on Keratinocytes in Vitro
Markus Denzinger
orcid.org/0000-0003-1580-0273
; Department of Pediatric Surgery, Klinik St. Hedwig, University Medical Center Regens- burg, Regensburg, Germany
Andreas K. Nüssler
; Siegfried Weller Institute for Trauma Re- search, Eberhard-Karls-Universität, Tübingen, Germany
Fabian Medved
; Clinic for Plastic, Re- constructive, Hand and Burn Surgery, BG Trauma Center, University of Tübingen, Tübingen, Germany
Sažetak
ABSTRACT
Background: This study examined the effects of irradiation with blue
light on HaCaT keratinocytes. As irradiation with blue light is known to be
antimicrobial, it offers a promising alternative therapy for contaminated
wounds. There is evidence that red light promotes wound healing, but
the potential benefits of irradiation with blue light have not yet been ad-
equately investigated.
Methods: The rate of wound closure in sterile and contaminated cells
was measured using an in vitro scratch assay wound-healing model. Ad-
ditionally, cell viability after treatment was determined using a Sulforho-
damine B (SRB) assay.
Results: In both the sterile and contaminated groups, treated cells
showed delayed wound closure when compared with cells not irradiated
with blue light. Additionally, treatment with blue light resulted in poorer
viability in the treatment groups.
Conclusion: Although irradiation with blue light may offer a promising
alternative therapy for reducing bacterial colonization, our data indicate
that re-epithelization may be negatively influenced by blue light. Further
research is needed to clarify possible wound healing applications.
Ključne riječi
Hrčak ID:
311553
URI
Datum izdavanja:
15.6.2023.
Posjeta: 147 *