Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2023.2194
Purification of Hyodeoxycholic Acid and Chenodeoxycholic Acid from Pig Bile Saponification Solution Using Column Chromatography
B. Liu
; College of Pharmaceutical Engineering, Tianjin University of Traditional Chinese Medicine, Tianjin 300193
T. Chen
; Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
X. Chen
; Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
X. Li
; Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
K. Abudureheman
; Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
S. Hu
; Zhejiang Canaan iPharm-Technology Co.Ltd., Hangzhou, Zhejiang, 311100, China
X. Gong
; a) Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China; b) College of Pharmaceutical Engineering, Tianjin University of Traditional Chinese Medicine, Tianjin 300193; d) Jinhua Institute of Zhejiang University, Jinhua 321016, China
Sažetak
The purpose of this study was to purify hyodeoxycholic acid and chenodeoxycholic acid from pig bile saponification solution using column chromatography. The optimal resin was selected through static adsorption experiments. Loading, washing, and elution conditions were optimized. Hyodeoxycholic acid and chenodeoxycholic acid were further purified through crystallization. The best resin was found to be CG161M macroporous resin. After optimization, the sample was loaded at a volume flow of 3 BV h–1 to
adsorb 5 BV, washed with 40 % ethanol, eluted with 45 % ethanol and 60 % ethanol, respectively. This resulted in purities for hyodeoxycholic acid of 70.34 % and 66.21 % for chenodeoxycholic acid with yields of 86.48 % and 90.57 %, respectively. Crystals of hyodeoxycholic acid with a purity of 91.04 % and chenodeoxycholic acid precipitate with a purity of 80.28 % were obtained through crystallization. Hyodeoxycholic acid and chenodeoxycholic acid can be purified using CG161M resin, and subsequent crystallization yields high-purity hyodeoxycholic acid.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
hyodeoxycholic acid; chenodeoxycholic acid; column chromatography; separation; purification
Hrčak ID:
313482
URI
Datum izdavanja:
19.1.2024.
Posjeta: 92 *