Original scientific paper
https://doi.org/10.15177/seefor.23-13
The Community of Fine Root Fungi of Silver Fir (Abies alba Mill.) Saplings
Marlena Baranowska
; Poznań University of Life Sciences, Faculty of Forestry and Wood Technology, Department of Silviculture, Wojska Polskiego 71A, PL-60-625 Poznań, Poland
Władysław Barzdajn
; Poznań University of Life Sciences, Faculty of Forestry and Wood Technology, Department of Silviculture, Wojska Polskiego 71A, PL-60-625 Poznań, Poland
Robert Korzeniewicz
; Poznań University of Life Sciences, Faculty of Forestry and Wood Technology, Department of Silviculture, Wojska Polskiego 71A, PL-60-625 Poznań, Poland
Wojciech Kowalkowski
; Poznań University of Life Sciences, Faculty of Forestry and Wood Technology, Department of Silviculture, Wojska Polskiego 71A, PL-60-625 Poznań, Poland
Adrian Łukowski
; Poznań University of Life Sciences, Faculty of Forestry and Wood Technology, Department of Silviculture, Wojska Polskiego 71A, PL-60-625 Poznań, Poland
Jolanta Behnke-Borowczyk
; Poznań University of Life Sciences, Faculty of Forestry and Wood Technology, Department of Entomology and Forest Pathology, Wojska Polskiego 71C, PL-60-625 Poznań, Poland
Mirzeta Memišević Hodžić
; University of Sarajevo, Faculty of Forestry, Department of Cultivation of Forests and Urban Greens, Zagrebačka 20, BA-71000 Sarajevo, Bosnia and Herzegovina
Abstract
This study aimed to assess the biodiversity of fungi colonizing the fine roots (diameter up to 2 mm) of 3-year-old silver fir saplings from areas of Międzylesie Forest District in Poland. It was hypothesized that quantitatively and qualitatively, mycorrhizal fungi would be the dominant fungi in root communities of silver fir. DNA extraction was performed using Plant Genomic DNA purification. The internal transcribed spacer1 (ITS1) rDNA region was amplified using specific primers, and the amplicons were purified and sequenced using sequencing by synthesis (SBS) Illumina technology. The obtained sequences were compared with reference sequences in the UNITE database (https://unite.ut.ee/) using the basic local alignment search tool (BLAST) algorithm to facilitate species identification. A total of 307,511 OTUs was obtained from each sample. There were 246,477 OTUs (80.15%) of fungi known from cultures. The genera Tuber spp. (7.51%) and Acephala spp. (3.23%) accounted for the largest share of the fungal communities on the fine roots of fir trees. Hence our results indicate the dominance of mycorrhizal fungi in these communities and reflect the excellent quality of the saplings that were assessed. Pathogenic fungi constituted a much smaller share of the fungal communities.
Keywords
Lower Silesia; Międzylesie Forest District; nursery; restoration; Sudeten Mountains
Hrčak ID:
313812
URI
Publication date:
2.9.2023.
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