Izvorni znanstveni članak

https://doi.org/10.31727/gzb.47.3.10

Propagation of miscantus plant by micropropagation procedure

Silvija Zeman ; Polytechnic of Međimurje in Čakovec, Čakovec, Croatia
Melita Srpak ; Varaždin County – Department of Physical Planning, Varaždin, Croatia

Sažetak

Miscanthus is a perennial grass native to East Asia. Although miscanthus primarily spread in Europe and North America for its use as an ornamental grass, growing this plant is financially demanding, resulting in a limited area of cultivation in Europe. The aim of this work is to describe in detail different micropropagation systems applied to the miscanthus plant. Miscanthus propagation is done vegetatively, using underground rhizomes or young plants grown by tissue culture, known as micropropagation. Micropropagation stands out as an alternative and extremely efficient method of reproduction, given the high rate of multiplication and the additional advantage of preventing disease transmission. It represents a method of vegetative reproduction that takes place on artificially created nutrient media, controlled growth conditions and in vitro sterile conditions. Research materials and methods were based on latent buds of three-year Miscanthus × giganteus rhizomes. A 70 percent ethanol solution and a 0.1 percent HgCl2 solution were used for sterilization. Micropropagation of the miscanthus plant is an important technique for breeding purposes in the future as it offers a diverse range of advantages compared to the limitations of common and natural reproductive barriers in conventional breeding techniques. The results of this research are based on the fact that the process ensures a quick process of obtaining a large number of plants that are identical in genetic potential, growth and development of the species. This paper is structured in such a way as to point out the importance of miscanthus production by micropropagation, its characteristics, current knowledge about this plant, the achievements, future and perspective of miscanthus micropropagation using the in vitro technique.

Ključne riječi

in vitro; miscanthus; plant; micropropagation; propagation; reproduction; sterile conditions

Hrčak ID:

316398

URI

https://hrcak.srce.hr/316398

Datum izdavanja:

14.4.2024.

Podaci na drugim jezicima: hrvatski

Posjeta: 15 *