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Stručni rad

https://doi.org/10.2478/10004-1254-59-2008-1903

Cytotoxicity of Polyphenolic/Flavonoid Compounds in a Leukemia Cell Culture

Pavle Josipović ; Zavod za animalnu fiziologiju, Biološki odsjek, Prirodoslovno-matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska
Nadica Oršolić orcid id orcid.org/0000-0001-5102-3606 ; Zavod za animalnu fiziologiju, Biološki odsjek, Prirodoslovno-matematički fakultet, Sveučilište u Zagrebu, Zagreb, Hrvatska


Puni tekst: hrvatski pdf 414 Kb

str. 299-308

preuzimanja: 1.005

citiraj


Sažetak

Flavonoid components of propolis are biologically active substances with antioxidative, immunostimulative, immunomodulative, and anti-inflamatory properties. The aim of the study was to investigate their cytotoxic effect on different leukaemia cell lines. For this purpose we used five different flavonoids (quercetin, caffeic acid, chrysin, naringenin, and naringin) and five types of leukemia cell lines (MOLT, JURKAT, HL-60, RAJI and U937). Cells were cultured at 37 °C in the RPMI-1640 medium supplemented with 10 % FCS in humified atmosphere with 5 % of CO2. Flavonoids were added in the following concentrations: 100 µg mL-1, 50 µg mL-1, 25 µg mL-1, or 12.5 µg mL-1. The results show different dose- and cell-type-dependent cytotoxicity. Among the flavonoids, quercetin showed the strongest cytotoxic effect in all cell lines. Caffeic acid and chrisyn also expressed a high level of cytotoxicty. Treatment of U937 and HL-60 cell lines with low concentrations of chrisyn or naringenin stimulated cell proliferation. These results suggest a biphase effect of the tested compounds on monocyte cell lines. Cytotoxicity and growth stimulation mechanisms caused directly by flavonoids should further be investigated on the molecular level.

Ključne riječi

caffeic acid, cell proliferation, chrisyn, HL-60, JURKAT, MOLT, naringenin, propolis, quercetin, RAJI, U937 cells

Hrčak ID:

29564

URI

https://hrcak.srce.hr/29564

Podaci na drugim jezicima: hrvatski

Posjeta: 2.014 *