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https://doi.org/10.46419/vs.56.5.11

Vaginal Cytology and Progesterone Level Correlations during Oestrous Cycle Monitoring in Female Dogs

Rédha Belala ; Biotechnologies Laboratory related to Animal Reproduction (LBRA), Institute of Veterinary Medicine, Saad Dahleb Blida University 1, Blida, Algeria, Biotechnologies Platform for Animal Medicine & Reproduction (BIOMERA), Saad Dahleb Blida University 1, Algeria
Seddik Kebbal ; Biotechnologies Laboratory related to Animal Reproduction (LBRA), Institute of Veterinary Medicine, Saad Dahleb Blida University 1, Blida, Algeria, Biotechnologies Platform for Animal Medicine & Reproduction (BIOMERA), Saad Dahleb Blida University 1, Algeria
Choayb Mecherouk ; Biotechnologies Laboratory related to Animal Reproduction (LBRA), Institute of Veterinary Medicine, Saad Dahleb Blida University 1, Blida, Algeria
Rachid Kaidi ; Biotechnologies Laboratory related to Animal Reproduction (LBRA), Institute of Veterinary Medicine, Saad Dahleb Blida University 1, Blida, Algeria, Biotechnologies Platform for Animal Medicine & Reproduction (BIOMERA), Saad Dahleb Blida University 1, Algeria, Animal Health and Production Laboratory (SPA), Higher National Veterinary School of Algiers, Algeria
Nora Mimoune orcid.org/0000-0002-0900-3908 ; Biotechnologies Laboratory related to Animal Reproduction (LBRA), Institute of Veterinary Medicine, Saad Dahleb Blida University 1, Blida, Algeria, Biotechnologies Platform for Animal Medicine & Reproduction (BIOMERA), Saad Dahleb Blida University 1, Algeria, Animal Health and Production Laboratory (SPA), Higher National Veterinary School of Algiers, Algeria *

* Dopisni autor.

Sažetak

This study investigated the relationship between vaginal cytological examination and progesterone levels (P4) during a 12.5-month period of cycle monitoring in 16 domestic bitches subjected to oestrus induction treatments. By analysing the correlations between P4 rates and cellular parameters (specifically eosinophilia and basophilia percentage), the study aimed to establish a reliable cellular parameter that can serve as an indicator of low progesterone thresholds. Preliminary monitoring was conducted through bi-weekly visits. At each visit, all animals systematically underwent a vaginal smear stained with the Harris-Schorr trichrome method. For P4 assess-ment, plasma samples were analysed using a radioimmunoassay method. Cycle control during and after oestrus induction was accompanied by clinical, cytological and hormonal monitoring until the gestational state was confirmed. Chi-square test was applied to study the correlation in a population consisting of 282 smear slides corresponding to the corre- sponding P4 values. Data showed that at the beginning of proestrus, P4 levels were highly significantly correlated with the percentages of basophilic and eosinophilic cells. Among the cytological criteria evaluated for both cellular percentages, having more than 40% basophilic cells on the vaginal smear proved to be the most reliable with a zero margin of error. Its application indicated that when the percentage of basophilic cells is strictly greater than 40%, P4 values are below 2 ng/mL, and once the percentage of basophilia falls below 40%, the P4 assays become valuable. Thus, in the context of cycle monitoring, this cellular indicator could be particularly useful in avoiding costly and unnecessary progesterone measurements and reducing their frequency at the beginning of proestrus.

Ključne riječi

oestrus monitoring; bitch; vaginal cytology; progesterone

Hrčak ID:

326192

URI

https://hrcak.srce.hr/326192

Datum izdavanja:

3.4.2025.

Podaci na drugim jezicima: hrvatski

Posjeta: 0 *