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https://doi.org/10.46419/vs.56.5.12

Dependence of Blood Biochemical Parameters on the Degree of Microstructural Changes in Chronic Pancreatitis in Dogs

Vyacheslav Samoilіuk orcid.org/0000-0001-8400-8904 ; Department of Veterinary Surgery and Reproduction, Faculty of Veterinary Medicine, State Agrarian and Economic University, Dnipro, Ukraine *
Mykhaylo Koziy ; Department of medical and biological foundations of sport and physical culture and sports rehabilitation, Faculty of physical education and sports, Petro Mohyla Black Sea National University, Mykolayiv, Ukraine
Oleksandr Semonov ; Department of Clinical Diagnostics and Internal Diseases of Animals, Faculty of Veterinary Medicine, State Agrarian and Economic University, Dnipro, Ukraine
Dmytro Bilyi ; Department of Veterinary Surgery and Reproduction, Faculty of Veterinary Medicine, State Agrarian and Economic University, Dnipro, Ukraine
Dmytro Slusarenko ; Department of Veterinary Surgery and Reproduction, Faculty of Veterinary Medicine, State Biotechnological University, Kharkiv, Ukraine
Sergiy Maslikov ; Department of Veterinary Surgery and Reproduction, Faculty of Veterinary Medicine, State Agrarian and Economic University, Dnipro, Ukraine

* Dopisni autor.

Sažetak

The study investigated the biochemical parameters of blood in comparison to histological findings in dogs with pancreatitis. Tissue samples were collected from the left and right lobes and the body of the pancreas during autopsy, followed by histological examination to assess the degree of inflammation, fibrosis, and necrotic changes. The severity of microstructural damage was classified into three grades based on the progression of fibrotic changes: mild, moderate and severe (characterised by dense pancreatic fibrosis). Histological changes in the pancreas in cases of chronic pancreatitis were compared to those in healthy dog pancreatic tissue obtained post-mortem from animals that died in traffic accidents. For each animal, the histological results were compared with the biochemical parameters of blood serum, measured prior to death during the diagnostic and treatment stages. In cases of mild microstructural changes, connective tissue proliferation occurred alongside minor atrophy of the exocrine component, with the structure of Langerhans-Sobolev islets preserved. Moder- ate damage involved more pronounced separation of exocrine parenchyma by fibrous tissue. Severe damage was characterised by acinar cell involvement, including vacuolar degeneration and atrophy. Loose fibrosis was typical for mild and moderate damage, while dense fibrosis was indicative of severe microstructural changes. Inflammation in the pancreas was not diffuse but was instead localised. The study established that the development of fibrosis plays a critical role in the pathogenesis of chronic pancreatitis. Blood biochemical parameters were correlated with the severity of pathological microstructural changes. Specifically, the activity of alkaline phosphatase increased by 1.3 times. In severe cases, alanine aminotransferase activity showed a significant increase compared to moderate and mild forms. Elevated levels of connective tissue metabolites in blood serum were identified as informative diagnostic markers for chronic pancreatitis of varying degrees of microstructural damage. These markers provide valuable insight into the diagnosis and progression of the disease.

Ključne riječi

pancreatic gland; fibrosis; histological alterations; investigation; degree of lesion; diagnostics

Hrčak ID:

326193

URI

https://hrcak.srce.hr/326193

Datum izdavanja:

3.4.2025.

Podaci na drugim jezicima: hrvatski

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