Original scientific paper

https://doi.org/10.2478/aiht-2025-76-3964

Oestrogenic and androgenic activity of oxybenzone and methylparaben in vitro

Nikolina Peranić ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Lucija Božičević ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Ivana Vinković Vrček ; Institute for Medical Research and Occupational Health, Zagreb, University of Rijeka Faculty of Medicine, Rijeka, Croatia

Abstract

Motivated by emerging concerns about health hazards associated with various industrial chemicals, this study investigated the disruption of endocrine system using well established in vitro assays. Due to the lack of scientific data on adverse effects of chemicals used in personal care products (PCPs), the focus was placed on oestrogenic and androgenic action of photostabiliser oxybenzone and preservative methylparaben. To this end we relied on in vitro assays for oestrogen and androgen receptor activation based on HeLa-9903 and AREcoScreen GR KO M1 cell lines to determine dose response according to respective OECD Test Guidelines 455 and 458. Our findings clearly demonstrate that both chemicals act as oestrogen receptor agonists and androgen receptor antagonists, raising additional concerns about health risks for humans posed by excessive and widespread use of such chemicals in PCPs.

Keywords

agonist; antagonist; AR-EcoScreen GR KO M1; endocrine disruption; HeLa-9903; hormone receptors; personal care products

Hrčak ID:

329438

URI

https://hrcak.srce.hr/329438

Publication date:

26.3.2025.

Article data in other languages: croatian

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