Original scientific paper
https://doi.org/10.3325/cmj.2023.64.391
4-phenyl butyric acid improves hepatic ischemia/reperfusion and affects gene expression of ABC transporter Abcc5 in rats
Bülent Barış Güven
orcid.org/0000-0002-3628-7408
; Department of Anesthesiology, Dr. Suat Gunsel University of Kyrenia Hospital, Kyrenia, Cyprus
*
Alpaslan Tanoglu
; Gastroenterology Division, Department of Internal Medicine, Bahcesehir University Faculty of Medicine, Istanbul, Turkey
Fatih Ozcelik
; Department of Medical Biochemistry, University of Health Sciences Turkey, Hamidiye Etfal Training Hospital, Istanbul, Turkey
Esra Guzel Tanoglu
; Department of Molecular Biology and Genetics, University of Health Sciences, Istanbul, Turkey
Neslihan Kaya Terzi
; Department of Pathology, Canakkale Onsekiz Mart University, Faculty of Medicine, Canakkale, Turkey
* Corresponding author.
Abstract
Aim To assess the effects of 4-phenyl butyric acid (PBA) on
oxidative stress, inflammation, liver histology, endoplasmic
(ER) reticulum stress, and the expression levels of ATP-binding cassette transporter family members in a hepatic ischemia-reperfusion (IR) model.
Methods Thirty-five rats were randomly divided into five
groups: sham, IR, IR + 100 mg kg−1 PBA, IR + 200 mg kg−1
PBA, and IR + placebo. After sacrifice, we assessed serum
biochemical variables, myeloperoxidase (MPO), malondialdehyde (MDA), total antioxidant status (TAS), and total oxidant status (TOS). The expression levels of Abcc (2 and 5),
Abcg2, Abcf2, Ire1-α, and Perk genes were measured with a
quantitative real-time polymerase chain reaction.
Results Serum biochemical variables, MPO, MDA, TAS,
and TOS levels of the PBA groups (especially in the low
dose group) were lower than in the IR and placebo group
(P<0.05). Histological tissue damage in the IR group was
more severe than in the PBA groups. Ire1-α and Perk expression levels were significantly lower in the PBA groups
than the IR group (P<0.001). Abcc (2 and 5) and Abcg2
expression levels were significantly lower in the IR group
than in the sham and PBA groups (P<0.001, P<0.035, and
P<0.009, respectively).
Conclusions The use of PBA significantly positively affected IR injury, which makes PBA a candidate treatment to
reduce liver IR.
Keywords
Hrčak ID:
331884
URI
Publication date:
21.12.2023.
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