Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2024.65.232
Strong association of TLR2 and TLR3 polymorphisms with keratoacanthoma and common warts: a case-control study
Silvana Karabatić Knezović
; Secondary Medical School Split, Split, Croatia
Dora Knezović
; Laboratory for cancer research, University of Split School of Medicine, Split, Croatia
Antonela Matana
; Department of Health Studies, University of Split, Split, Croatia
Neira Puizina Ivić
; Department of Dermatovenerology, University Hospital of Split, Split, Croatia
Irena Drmić Hofman
; Department of Health Studies, University of Split, Split, Croatia
*
* Dopisni autor.
Sažetak
Aim To determine variations in allele and genotype frequencies between keratoacanthoma (KA) and common
warts (CW), compared with the control group, in three
single nucleotide polymorphisms (SNPs) within the TLR2,
TLR3, and TLR9 genes.
Methods This case-control study involved samples from
161 patients with KA, 152 patients with CW, and 469 controls. DNA was isolated from formalin-fixed paraffin-embedded tissue sections. Three SNPs - rs4696480 in TLR2,
rs7657186 in TLR9, and rs35213 in TLR3 - were genotyped
with TaqMan Genotyping Assays on the 7500 Real-Time
PCR System.
Results TLR2 rs4696480 and TLR3 rs7657186 were significantly overrepresented in KA and CW compared with controls (P<0.001). The association was stronger for CW than
for KA, as evidenced by higher frequencies of the A allele
and AA genotype for rs4696480. Both KA and CW patients
had higher frequencies of the G allele and GG genotype for
rs7657186 than controls. rs7657186 was moderately associated with KA and CW, with the G allele and GG genotype
being more prevalent in CW cases, where no AA homozygotes were found.
Conclusion Genetic variants in TLR2 (rs4696480) and TLR3
(rs7657186) genes may affect KA and CW development, influencing immune responses and susceptibility to these
skin lesions. Further research is required to elucidate TLR
expression patterns and their role in KA development.
Ključne riječi
Hrčak ID:
332023
URI
Datum izdavanja:
16.6.2024.
Posjeta: 35 *