Periodicum biologorum, Vol. 110 No. 3, 2008.
Izvorni znanstveni članak
Growth habit and photosynthetic activity of shoot cultures of Medicago sativa L. transformed with the oryzacystatin II gene
BRANKA UZELAC
; Institute for Biological Research, »Siniša Stanković«, Bulevar despota Stefana 142, 11060 Belgrade, Serbia
ZORICA POPOVIĆ
; Institute for Biological Research, »Siniša Stanković«, Bulevar despota Stefana 142, 11060 Belgrade, Serbia
ALEKSANDAR MIJOVIĆ
; Environment Protection Agency, Ministry of Environment and Spatial Planning, Ru`e Jovanovi}a 27a, 11060 Belgrade, Serbia
SNEŽANA BUDIMIR
; Institute for Biological Research, »Siniša Stanković«, Bulevar despota Stefana 142, 11060 Belgrade, Serbia
DUŠICA JANOŠEVIĆ
; Institute of Botany and Botanical Garden »Jevremovac«, Faculty of Biology, University of Belgrade, Takovska 43, Belgrade, Serbia
ANN SMIGOCKI
; USDA-ARS, Molecular Plant Pathology Laboratory, Beltsville, MD 20705, USA
SLAVICA NINKOVIĆ
; Institute for Biological Research, »Siniša Stanković«, Bulevar despota Stefana 142, 11060 Belgrade, Serbia
Sažetak
Abstract
Background and Purpose: Introduction of a foreign gene into plant genome may induce morphological and physiological alterations in transgenic plants. The purpose of this study was to assess the impact of gene introduction on morphology and photosynthetic activity of two transgenic alfalfa shoot cultures.
Material and Methods: In vitro maintained shoot cultures of alfalfa (Medicago sativa L. cv. Zaječarska 83) that were transformed with the oryzacystatin II (OC-II) gene and propagated on growth regulator-free medium were subjected to analysis of morphological characteristics and photosynthetic activity. For analysis of phological characteristics, the length of the main axis, the number of axillary and adventitious shoots, total number of leaves and the number of senesced leaves as well as plant dry mass were determined. Net photosynthetic rate was determined as CO2 influx using a LI-6200 closed photosynthesis system. Photosynthetic function was assessed as the rate of basic chlorophyll fluorescence and determined with a Plant Stress Meter by method of induced fluorometry. Chlorophyll content in leaf samples was determined spectrophotometrically.
Results: The most striking feature of transformed cultures was reduced apical dominance and the absence of adventitious roots. In comparisonwith control, main axis length was also reduced. In addition, a general decrease in photosynthesis in transgenic shoots was also observed.
Conclusion: Both morphology and the key processes in photosynthesis were modified in transgenic shoots.However, the fact that transgenic plants carry a gene that confers pest resistance gives potential agronomic value to at least some of the clones whose morphological characteristics and photosynthetic acivity were minimally disturbed.
Ključne riječi
alfalfa; chlorophyll fluorescence; CO2 influx; genetic transformation; Medicago sativa; morphology; oryzacystatin II
Hrčak ID:
32570
URI
Datum izdavanja:
31.10.2008.
Posjeta: 1.510 *