Izvorni znanstveni članak
Prognostic Significance of B-cell Differentiation Genes Encoding Proteins in Diffuse Large B-cell Lymphoma and Follicular Lymphoma Grade 3
Ana Borovečki
; Department of Clinical Pathology and Cytology Merkur University Hospital, Zagreb Croatia
Petra Korać
; Department of Clinical Pathology and Cytology Merkur University Hospital, Zagreb Croatia
Marin Nola
; Department of Pathology. Zagreb University Hospital Center, Zagreb, Croatia
Davor Ivanković
; Andrija Štampar School of Public Health, Zagreb, Croatia
Branimir Jakšić
; University Department of Internal Medicine, Merkur University Hospital, Zagreb, Croatia
Mara Dominis
; Department of Clinical Pathology and Cytology Merkur University Hospital, Zagreb Croatia
Sažetak
Aim To define prognostic significance of B-cell differentiation genes encoding
proteins and BCL2 and BCL6 gene abnormalities in diffuse large B-cell lymphoma
and follicular lymphoma grade 3 with >75% follicular growth pattern.
Methods In 53 patients with diffuse large B-cell lymphoma and 20 patients
with follicular lymphoma grade 3 with >75% follicular growth pattern the
following was performed: 1) determination of protein expression of BCL6,
CD10, MUM1/IRF4, CD138, and BCL2 by immunohistochemistry; 2) subclassification
into germinal center B-cell-like (GCB) and activated B-cell-like
(ABC) groups according to the results of protein expression; 3) detection of
t(14;18)(q32;q21)/IgH-BCL2 and BCL6 abnormalities by fluorescent in situ
hybridization in diffuse large B-cell lymphoma and follicular lymphoma grade
3 with >75% follicular growth pattern as well as in GCB and ABC groups; and
4) assessment of the influence of the analyzed characteristics and clinical prognostic
factors on overall survival.
Results Isolated BCL6 expression was more frequently found in follicular lymphoma
grade 3 with >75% follicular growth pattern than in diffuse large Bcell
lymphoma (P = 0.030). There were no differences in BCL2 and BCL6 gene
abnormalities between diffuse large B-cell lymphoma and follicular lymphoma
grade 3 with >75% follicular growth pattern. Diffuse large B-cell lymphoma and
follicular lymphoma grade 3 with >75% follicular growth pattern patients were
equally distributed in GCB and ABC groups. t(14;18)(q32;q21) was more frequently
recorded in GCB group, and t(14;18)(q32;q21) with BCL2 additional
signals or only BCL2 and IgH additional signals in ABC group (P = 0.004).
The GCB and ABC groups showed no difference in BCL6 gene abnormalities.
There was no overall survival difference between the patients with diffuse large
B-cell lymphoma or follicular lymphoma grade 3 with >75% follicular growth
pattern; however, GCB group had longer overall survival than ABC group
(P = 0.047). Multivariate analysis showed that BCL6, CD10, and BCL2 expression,
BCL2 and BCL6 abnormalities, and International Prognostic Index were
not significantly related to overall survival.
Conclusion Diffuse large B-cell lymphoma and follicular lymphoma grade 3
with >75% follicular growth pattern patients have very similar characteristics
and their prognosis is more influenced by protein expression of B-cell differentiation
stage genes than by tumor cells growth pattern, BCL2 and BCL6 abnormalities,
and International Prognostic Index.
Ključne riječi
DLBCL; FL-3; GCB; ABC; BCL2; BCL6
Hrčak ID:
35099
URI
Datum izdavanja:
15.10.2008.
Posjeta: 1.275 *