Izvorni znanstveni članak
Association of Estrogen Receptor α Gene Polymorphisms with Cytokine Genes Expression in Systemic Lupus Erythematosus
Zhi-Ming Lu
; Department of Laboratory Medicine, Shandong Provincial Hospital, People's Republic of China
Zi-E Wang
; Department of Laboratory Medicine, Shandong Provincial Hospital, People's Republic of China
Yi-Qing Liu
; Department of Laboratory Medicine, Shandong Provincial Hospital, People's Republic of China
Chun-Xiao Wu
; Department of Laboratory Medicine, Shandong Provincial Hospital, People's Republic of China
Chang-Yin Wang
; Department of Laboratory Medicine, Shandong Provincial Hospital, People's Republic of China
Bing-Chang Zhang
; Department of Laboratory Medicine, Shandong Provincial Hospital, People's Republic of China
Song Shao
; Department of Laboratory Medicine, Shandong Provincial Hospital, People's Republic of China
Yu-Lian Jiao
; Research Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan, People's Republic of China
Zhi-Xiang Che
; Department of Laboratory Medicine, Shandong Provincial Hospital, People's Republic of China
Zi-Jiang Chen
; Research Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan, People's Republic of China
Yue-Ran Zhao
; Central Laboratory, Shandong Provincial Hospital, Shandong University, Jinan, People's Republic of China
Sažetak
Aim To analyze the association of estrogen receptor α
(ORα) gene polymorphisms with cytokine genes expression
in patients with systemic lupus erythematosus (SLE)
and controls.
Methods Genomic DNA was extracted and polymorphisms
of XbaІ (XX, Xx, or xx genotype) and PvuII (PP, Pp, or
pp) in intron 1 of ORα gene were detected by polymerase
chain reaction-restriction fragment length polymorphisms
(PCR-RFLP) method. The messenger RNA (mRNA) levels
of interleukin (IL)-10, IL-4, interferon (IFN)-γ, and IL-2 were
assessed by semiquantitative reverse transcription polymerase
chain reaction (RT-PCR).
Results In patients with SLE with PpXx genotype, IL-10 and
IL-4 mRNA expression was higher (P < 0.001 and P = 0.013,
respectively), while in patients with SLE with Ppxx genotype
IFN-γ and IL-2 mRNA expression was lower than in
controls (P < 0.001). There was no significant difference in
mRNA expression of 4 cytokines among controls with various
genotypes.
Conclusion ORα gene polymorphism may be associated
with the expression of IL-10, IL-4, IL-2, and IFN-γ in patients
with SLE.
Ključne riječi
oestrogen receptor α; cytokine; IL-10; IFN-γ; IL-4; IL-2
Hrčak ID:
38312
URI
Datum izdavanja:
15.4.2009.
Posjeta: 1.300 *