Izvorni znanstveni članak
Kinship and Y-Chromosome Analysis of 7th Century Human Remains: Novel DNA Extraction and Typing Procedure for Ancient Material
Daniel Vanek
; Forensic DNA Service, Prague, Czech Republic
Lenka Saskova
; Forensic DNA Service, Prague, Czech Republic
Hubert Koch
; Bavarian State Department of Monuments and Sights, Regensburg, Germany
Sažetak
Aim To develop novel DNA extraction and typing procedure
for DNA identification of the 7th century human remains,
determine the familiar relationship between the
individuals, estimate the Y-chromosome haplogroup, and
compare the Y-chromosome haplotype with the contemporary
populations.
Methods DNA from preserved femur samples was extracted
using the modified silica-based extraction technique.
Polymerase chain reaction amplification was performed
using human identification kits MiniFiler, Identifiler,
and Y-filer and also laboratory-developed and validated Ychromosome
short tandem repeat (STR) pentaplexes with
short amplicons.
Results For 244A, 244B, 244C samples, full autosomal DNA
profiles (15 STR markers and Amelogenin) and for 244D,
244E, 244F samples, MiniFiler profiles were produced. Ychromosome
haplotypes consisting of up to 24 STR markers
were determined and used to predict the Y-chromosome
haplogroups and compare the resulting haplotypes
with the current population. Samples 244A, 244B, 244C,
and 244D belong to Y-chromosome haplogroup R1b and
the samples 244E and 244F to haplogroup G2a. Comparison
of ancient haplotypes with the current population
yielded numerous close matches with genetic distance
bellow 2.
Conclusion Application of forensic genetics in archaeology
enables retrieving new types of information and helps
in data interpretation. The number of successfully typed
autosomal and Y-STR loci from ancient specimens in this
study is one of the largest published so far for aged samples.
Ključne riječi
ancient DNA; extraction; inhibitor; haplogroup; Y-chromosome; archaeogenetics; STR
Hrčak ID:
40681
URI
Datum izdavanja:
15.6.2009.
Posjeta: 1.762 *