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The beginning of human life under time-laps Cinematography

YASUYUKI MIO ; Reproductive Center, Mio Fertility Clinic 1-1, 2-Chome, Kuzumo-Minami, Yonago, Tottoriken, 683-0008 Japan, Kazuo Maeda, Dept. of Obstetrics and Gynecology (emeritus), Tottori University School of Medicine, Nadamachi 3-125, Yonago, Tottoriken, 683-0835 Jap

Sažetak

Background and Purpose: The aim of this study was to follow and record the early stages of development of a human embryo using time-lapse cinematography, and to discuss the beginning of human life.

Materials and Methods: Human ova were incubated in thermo-stabilized and pH controlled chambers and observed using an inverted microscope fitted with a CCD digital camera. Images were taken at 2-minute intervals for 5–6 days. A total of 30 consecutive images, corresponding to an hour of incubation, could then be replayed in 1 second. This allowed us to watch the development of embryos over 5–6 days in a few minutes.

Results and Conclusion: We observed fertilization of an ovum with a
single spermatozoon followed by early cleavages, formation of the morula, blastocyst hatching and changes in the embryonic plates and the development of monozygotic twins in 2 of 3 incubated blastocysts. TLC has contributed enormously to our understanding of the morphological mechanisms of fertilization and embryonic development.We would like to suggest that the time at which stem cell-like morula cells form the differentiated blastocyst is the point at which human life begins, and that the ET of blastocyst may increase the risk of monozygotic twins.

Ključne riječi

Embryo; blastocyst; human life; ART; time-laps cinematography; MD twins

Hrčak ID:

43353

URI

https://hrcak.srce.hr/43353

Datum izdavanja:

15.9.2009.

Posjeta: 1.569 *