Medicina, Vol. 45 No. 3, 2009.
Pregledni rad
Hyphenated techniques liquid chromatography-mass spectrometry: basic methodology and applicati ons
Mario Cindrić
; Insti tut “Ruđer Bošković”, Zavod za molekularnu medicinu, Laboratorij za sistemsku biomedicinu,, Zagreb, Hrvatska
Ana Marković
; Zavod za biokemiju, Kemijski odsjePrirodoslovno-matemati čk ifakultet, Sveučilište u Zagrebu, Zagreb, Hrvatskak,
Anita Horvatić
; Insti tut “Ruđer Bošković”, Zavod za molekularnu medicinu, Laboratorij za sistemsku biomedicinu,, Zagreb, Hrvatska
Sažetak
Liquid chromatography (LC) is a basic versati le separati on technique widely used in modern life sciences especially in the fi elds closely related to molecular biology, namely in the analytical or preparative chemistry. Unlike many other separati on techniques, which are unsuitable for thermally degradable molecules (e.g. gas chromatography, GC), or which are not feasible for on-line coupling (e.g. isoelectric focusing, IEF), liquid chromatography can efficiently separate a very wide range of large molecules like polymers, peptides and proteins as well as small molecules like drug or drug metabolites. Choosing the proper LC method that uti lize mass spectrometry (MS) friendly conditi ons (volati le buff ers, stable
and low fl ow rate, polar organic solvents etc.), liquid chromatography system can be easily hyphenated to mass spectrometer. Ionizati on source, analyzer (or combinati on of
more analyzers in one instrument, the so called tandem mass spectrometry) and ion detector are selected according to the analysis requirements that might range from simple one ion qualitative analysis to substructural qualitati ve, and quanti tati ve analysis of
complex mixtures. To facilitate elucidati on of structure or quanti tati ve analysis different soft ware applicati ons and data bases search engines were developed during the last decade.
Ključne riječi
analyzer; data base; ionization; liquid chromatography; tandem mass spectrometry
Hrčak ID:
43663
URI
Datum izdavanja:
1.9.2009.
Posjeta: 6.581 *