Izvorni znanstveni članak
Alteration in TWIST Expression: Possible Role in Paclitaxelinduced Apoptosis in Human Laryngeal Carcinoma Hep-2 Cell Line
Liang Yu
; Department of Otolaryngology Head and Neck Surgery, Provincial Hospital affiliated to Shandond University, Jinan, People's Republic of China
Hui-zheng Li
; Department of Otolaryngology Head and Neck Surgery, Provincial Hospital affiliated to Shandond University, Jinan, People's Republic of China
Su-mei Lu
; Institute of Eye and Otolaryngology, Shandon Clinic Research Institute, Jinan, People's Republic of China
Wen-wen Liu
; Institute of Eye and Otolaryngology, Shandon Clinic Research Institute, Jinan, People's Republic of China
Jian-feng Li
; Institute of Eye and Otolaryngology, Shandon Clinic Research Institute, Jinan, People's Republic of China
Hai-bo Wang
; Department of Otolaryngology Head and Neck Surgery, Provincial Hospital affiliated to Shandond University, Jinan, People's Republic of China
Wei Xu
; Department of Otolaryngology Head and Neck Surgery, Provincial Hospital affiliated to Shandond University, Jinan, People's Republic of China
Sažetak
Aim To explore the relationship between alteration in the
expression of TWIST, highly conserved transcription factor
from the basic helix-loop-helix family, and apoptosis
of Hep-2 cells induced by chemotherapeutic agent paclitaxel.
Methods Morphological changes of Hep-2 cells were observed
by acridine orange cytochemistry staining. Viability
of Hep-2 cells treated with various concentrations of paclitaxel
was examined by cell proliferation assay. Apoptosis
was examined by flow cytometry. The mRNA and protein
expression of TWIST in response to paclitaxel at 24 hours,
48 hours, and 72 hours was examined by reverse transcription-
polymerase chain reaction (RT-PCR) and Western blotting,
respectively.
Results Typical morphological changes of apoptotic cells
at 24 hours, 48 hours, or 72 hours after treatment wiyth
paclitaxel (10 × 10−9 mol/L) were observed. The cell survival
rates significantly decreased in a concentration- and timedependent
manner (P = 0.001). Paclitaxel-induced apoptosis
increased with culture time (22.6 ± 5.3% after 24 hours,
38.7 ± 7.9% after 48 hours, and 52.4 ± 14.3% after 72 hours;
P = 0.002). Both mRNA and protein expression of TWIST
was markedly decreased at both mRNA levels and protein
levels, at 24 hours, 48 hours, and 72 hours in the paclitaxelinduced
apoptosis of Hep-2 cells (P < 0.001).
Conclusion TWIST, which has a significantly decreased expression
in response to paclitaxel in Hep-2 cells, may play a
pivotal role in paclitaxel-induced apoptosis of Hep-2 cells.
Ključne riječi
Transcription factor; TWIST; Hep-2 cells; Paclitaxel; Apoptosis; Laryngeal carcinoma; Chemotherapy
Hrčak ID:
47908
URI
Datum izdavanja:
15.12.2009.
Posjeta: 1.174 *