Izvorni znanstveni članak

Novel Kinetic Spectrophotometric Method for Determination of Tiopronin [N-(2-Mercaptopropionyl)-glycine]

Lea Kukoc-Modun orcid.org/0000-0003-1798-6796 ; Faculty of Chemistry and Technology, University of Split, Teslina 10/V, 21000 Split, Croatia
Njegomir Radić ; Faculty of Chemistry and Technology, University of Split, Teslina 10/V, 21000 Split, Croatia

Sažetak

Novel simple kinetic spectrophotometric method for the determination of tiopronin [N-(2-mercaptopropionyl)-glycine, MPG] in pharmaceutical formulation has been developed and validated. The
proposed method is based on the coupled redox-complexation reaction whose first step is reduction of
FeIII by MPG, while second one includes the complexation of FeII, resulted from preceding redox reaction,
with 2,4,6-trypyridyl-s-triazine (TPTZ). The stable Fe(TPTZ)22+ complex exhibits an absorption maximum
at  = 593 nm. The initial rate and fixed time (at 3 min) methods were utilized for constructing the calibration graphs.
The graphs were linear in concentration ranges from 1.0  10−6 to 1.0  10−4 mol L−1 for both methods with
limits of detection 1.3  10−7 mol L−1 and 7.5  10−8 mol L−1 for the initial rate and fixed time method, respectively.
The proposed methods were successfully applied for the determination of MPG in its commercial
pharmaceutical formulation.

Ključne riječi

tiopronin; N-(2-mercaptopropionyl)-glycine; kinetic spectrophotometry; initial rate method; fixed time method; pharmaceutical analysis

Hrčak ID:

56022

URI

https://hrcak.srce.hr/56022

Datum izdavanja:

15.7.2010.

Posjeta: 1.940 *