Izvorni znanstveni članak
Evaluation of Competitive ELISA for Detection of Antibodies to Brucella Infection in Domestic Animals
Lorraine L. Perrett
; Department of Statutory and Exotic Bacteria, Veterinary Laboratories Agency, Woodham Lane, Weybridge, United Kingdom
John A. McGiven
; Department of Statutory and Exotic Bacteria, Veterinary Laboratories Agency, Woodham Lane, Weybridge, United Kingdom
Simon D. Brew
; Department of Statutory and Exotic Bacteria, Veterinary Laboratories Agency, Woodham Lane, Weybridge, United Kingdom
Judith A. Stack
; Department of Statutory and Exotic Bacteria, Veterinary Laboratories Agency, Woodham Lane, Weybridge, United Kingdom
Sažetak
Aim To evaluate competitive enzyme-linked immunosorbent
assay (cELISA) for its suitability as an additional serological
test for the diagnosis of animal brucellosis.
Methods cELISA, which was developed at the Veterinary
Laboratories Agency, has been evaluated for its accuracy
and suitability as an additional serological test for the diagnosis
of animal brucellosis. Samples from naturally and experimentally
infected animals and those from Brucella-free
flocks and herds were tested.
Results Data obtained since 1991 were analyzed from routine
surveillance, animals experimentally infected with Brucella,
and stored sera to validate cELISA for the detection of
antibodies to Brucella in cows, small ruminants, and pigs.
The sensitivity of the test ranged from 92.31% to 100%, in
comparison with 77.14% to 100% for the complement fixation
test (CFT). Specificities for cELISA, indirect enzymelinked
immunosorbent assay, and CFT were greater than
90%.
Conclusion cELISA can be used on a variety of animal species,
and an added advantage is its suitability for use on
poor-quality samples such as those affected by hemolysis.
Ključne riječi
Brucella; Complement Fixation Test; Rose Bengal Test; Enzyme-linked immunosorbent assay
Hrčak ID:
58582
URI
Datum izdavanja:
15.8.2010.
Posjeta: 1.813 *