Veterinarski arhiv, Vol. 80 No. 5, 2010.
Izvorni znanstveni članak
Identification of avian species using polymerase chain reaction and sequence analysis of mitochondrial 12S rRNA gene
Nagappa S. Karabasanavar
; Department of Veterinary Public Health, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Patnagar, Uttarkhand, India
Suresh P. Singh
; Department of Veterinary Public Health, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Patnagar, Uttarkhand, India
Sunil N. Shebannavar
; Gennova Biopharmaceutical Pvt. LTD. Pune, Maharashtra, India
Angadi M. Kotresh
; Department of Veterinary Biochemistry, Veterinary College, Karnataka Veterinary, Animal and Fishery Sciences University (KVAFSU) Shivamogga, Karnataka, India
Patil S. Girish
; NRC on Meat, Indian Council of Agricultural Research, Hyderabad, Andra Pradesh, India
Pillai V. Umapathi
; Indian Council of Agricultural Research, Palampur, Himachal Pradesh, India
Sažetak
Authentic identification and differentiation of avian species is a vital step in conservative, taxonomic, forensic, legal and other ornithological interventions. The present investigation involved the application of molecular biological approach to identify and differentiate avian species i.e. two species of birds, namely black kite (Milvus migrans) and parakeet (Psittacula krameri). The DNA was isolated from blood samples of each species and a part of the mitochondrial 12S rRNA gene was amplified through polymerase chain reaction (PCR). The PCR products were sequenced and aligned using Basic Local Alignment Search Tool (BLAST) of the GenBank (NCBI). Based on the alignment and similarity/divergence, these avian species were accurately identified and differentiated.
Ključne riječi
12S rRNA gene; polymerase chain reaction; sequence analysis; avian species of mitochondrial 12S rRNA gene
Hrčak ID:
62159
URI
Datum izdavanja:
26.10.2010.
Posjeta: 1.430 *