Original scientific paper

Partitioning of Phenylalanine Ammonia-lyase from Rhodotorula glutinis in Aqueous Two-phase Systems of PEG/salts

WY. Wang ; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China
HY. Yue ; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China
QP. Yuan ; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China
WC. Wang ; College of Chemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, China

Abstract

In this work, the partitioning of phenylalanine ammonia-lyase was investigated in different systems of PEG1000, PEG2000, PEG3350, PEG6000 and PEG8000 with (NH4)2SO4, Na2SO4, Na2CO3 and potassium phosphate. The results showed that the partition of phenylalanine ammonia-lyase in PEG/salt aqueous two-phase systems was mainly influenced by hydrophobicity, excluded volume effect and salting-out effect; compared with other PEG/salt systems, PEG1000/Na2SO4 was the most effective for phenylalanine ammonia-lyase partitioning. The result further supports that PEG/salt
aqueous two-phase system provides a new and applicable route for the purification of phenylalanine ammonia-lyase, as shown in our published paper.13

Keywords

Aqueous two-phase system; phenylalanine ammonia-lyase; partitioning; PEG; salt

Hrčak ID:

62716

URI

https://hrcak.srce.hr/62716

Publication date:

27.12.2010.

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