Pregledni rad

Sequence Determination of Pharmaceutical Peptides by MALDI-TOF Tandem Mass Spectrometry

Mario Cindrić ; Institut Ruđer Bošković, Odjel za molekularnu medicinu, Zagreb, Hrvatska
Mirela Sedić ; Institut Ruđer Bošković, Odjel za molekularnu medicinu, Zagreb, Hrvatska
Anita Horvatić ; Institut Ruđer Bošković, Odjel za molekularnu medicinu, Zagreb, Hrvatska
Ivana Dodig ; Institut Ruđer Bošković, Odjel za molekularnu medicinu, Zagreb, Hrvatska

Sažetak

Although the peptide ion mechanisms of pre-dissociation, dissociation and post-dissociation have been well-studied over the past fifteen years, their practical application still has to be implemented into modern mass spectrometry-driven proteomics and bioanalysis. Unambiguous peptide sequence determination by mass spectrometry relies on the idea that only one continuous series of ions in mass spectrum can assure full sequence determination and meets the requirements of peptide analysis quality. This set of rules defined for the peptide analysis by tandem mass spectrometry would generally improve an overall reliability and data accuracy. Based on the process mechanisms of gas-phase peptide bond dissociation, a relatively small and large model peptides are unambiguously analyzed (bivalirudin and exenatide) showing that derivatization concepts of the C- or N-terminus derivatization (SPITC and Lys-tag) can be avoided.

Ključne riječi

MALDI-TOF/TOF; sequence determination; pharmaceutical peptide; bivalirudin; exenatide

Hrčak ID:

63153

URI

https://hrcak.srce.hr/63153

Datum izdavanja:

15.12.2010.

Podaci na drugim jezicima: hrvatski

Posjeta: 3.660 *