Veterinarski arhiv, Vol. 81 No. 1, 2011.
Izvorni znanstveni članak
Microsatellite BM1500 polymorphism and milk production traits in Vechur and crossbred cattle of Kerala.
Francis A. Lali
; College of Veterinary and Animal Sciences, Mannuthy, Kerala, India
Kannanbanat A. Bindu
; College of Veterinary and Animal Sciences, Mannuthy, Kerala, India
Sažetak
Vechur (Bos indicus) is the only native cattle breed of Kerala. The present study was aimed at leptin gene polymorphisms and their associations with milk production traits in 74 heads of Vechur and 108 crossbred cattle (Bos taurus × nondescript cattle) from Kerala. Microsatellite analysis was performed at the BM1500 marker, located 3.6 kb downstream from the leptin gene by polymerase chain reaction and denaturing polyacrylamide gel electrophoresis (6%). The alleles for the marker BM1500 in Vechur cattle were 126 bp, 132 bp, 136 bp, 144 bp and 150 bp whereas in crossbreds, the alleles were 126 bp, 132 bp, 136 bp, 138 bp and 144 bp. The 132 bp allele was the most abundant in Vechur and the crossbred cattle population with frequencies of 0.40 and 0.46, respectively. The 138 bp allele was absent in Vechur as against 150 bp allele in crossbreds. In comparison to earlier studies on BM1500 locus in cattle, two novel alleles of 126 and 132 bp were detected in the present study.
Allele frequency was highest for 132 bp in Vechur (0.40) as well as the crossbred cattle (0.46) under study. The polymorphic information content (PIC) of BM1500 marker in Vechur was 0.6595 while 0.656 in crossbred cattle. In the association study, the highest averages of 305 day milk yield and daily milk yield were observed for Vechur and crossbred cattle possessing 136 bp allele. The highest milk fat percentages were recorded in 136 bp allele bearing Vechur animals and crossbreds possessing 138 bp allele.
Ključne riječi
Vechur; leptin gene polymorphism; crossbred cattle of Kerala; BM1500 marker; milk production
Hrčak ID:
66536
URI
Datum izdavanja:
21.2.2011.
Posjeta: 1.325 *