Original scientific paper

https://doi.org/10.5562/cca2161

Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine Based on the Reduction of Copper(II)-neocuproine Reagent

Njegomir Radić ; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Teslina 10/V, HR-21000 Split, Croatia
Lea Kukoc-Modun orcid.org/0000-0003-1798-6796 ; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Teslina 10/V, HR-21000 Split, Croatia
Maja Biocic orcid.org/0000-0002-2293-3480 ; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Teslina 10/V, HR-21000 Split, Croatia

Abstract

A novel simple kinetic spectrophotometric method for the determination of N-acetyl-L-cysteine
(NAC) has been developed and validated. The proposed method is based on the reduction of CuIIneocuproine
reagent to CuI-neocuproine with the analyte, in a Britton-Robinson buffer solution (pH =
3.0). The non-steady state absorbance of the formed CuI-neocuproine complex is measured at 458 nm.
The initial rate and fixed time (at 1 min) methods were utilized for constructing the calibration graphs.
The calibration curves for both methods were linear in concentration range from 6.0  10–7 to 8.0  10–5
mol L–1 with the limit of detection 1.7  10–7 mol L–1. The slope of the initial method calibration curve
(1.0181) confirmed the first order reaction. Both proposed methods were successfully applied for the determination
of NAC in its commercial pharmaceutical formulations. (doi: 10.5562/cca2161)

Keywords

N-acetyl-L-cysteine; Kinetic spectrophotometry; Initial rate method; Fixed time method; Pharmaceutical analysis

Hrčak ID:

101910

URI

https://hrcak.srce.hr/101910

Publication date:

3.5.2013.

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