Original scientific paper

https://doi.org/10.5562/cca2109

Applicability of Liquid Chromatography with Time-of-Flight Mass Spectrometric Detection for Determination of Cyanuric Acid in Soil

Sanja Stipičević orcid.org/0000-0002-2021-9435 ; Institute for Medical Research and Occupational Health, Ksaverska c. 2, Zagreb, HR-10000, Croatia
Gordana Mendaš orcid.org/0000-0002-2661-2465 ; Institute for Medical Research and Occupational Health, Ksaverska c. 2, Zagreb, HR-10000, Croatia
Marko Vuletić ; PLIVA-Research & Development Ltd., Prilaz baruna Filipovića 25, Zagreb, HR-10000, Croatia
Sanja Fingler ; Institute for Medical Research and Occupational Health, Ksaverska c. 2, Zagreb, HR-10000, Croatia
Vlasta Drevenkar orcid.org/0000-0002-7430-0431 ; Institute for Medical Research and Occupational Health, Ksaverska c. 2, Zagreb, HR-10000, Croatia

Abstract

Cyanuric acid (2,4,6-trihydroxy-1,3,5-triazine, CA) is a highly polar and hydrophilic degradation
product of symmetric triazine herbicides, fairly stable under environmental conditions. We investigated
the applicability of reversed-phase liquid chromatography coupled to time-of-flight mass spectrometry
(LC-ToF-MS) for qualitative and quantitative determination of CA in soils of different origin and pedological
characteristics. CA was ultrasonically extracted from soils either with a mixture of acetone and
n-hexane (volume ratio = 2 : 1) or with methanol and analysed on a LiChrospher 100 CN column with
ψ(acetonitrile,water) = 50 : 50 as the mobile phase for isocratic elution. The efficiency and selectivity of
LC-ToF-MS determination, using the electrospray ionisation in negative mode and selective monitoring of
deprotonated CA molecule (m/z 128), was evaluated with respect to injection volume, extracting solvent,
and possible soil matrix interferences. The advantage of LC-ToF-MS over conventional HPLC with UVdiode
array detection was the high ion detection selectivity minimising the interferences of coeluting species
and the unambiguous identification of CA in soil extracts by accurate mass measurements of deprotonated
molecule. (doi: 10.5562/cca2109)

Keywords

cyanuric acid; soil, liquid chromatography; time-of-flight mass spectrometry; soil matrix interferences

Hrčak ID:

101913

URI

https://hrcak.srce.hr/101913

Publication date:

3.5.2013.

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