Veterinary Archives, Vol. 83 No. 3, 2013.
Original scientific paper
A study of Edwardsiella tarda colonizing live Asian clam, Corbicula fluminea, from Pasir Mas, Kelantan, Malaysia with the emphasis on its antibiogram, heavy metal tolerance and genetic diversity.
Lee Seong Wei
orcid.org/0000-0002-4854-2621
; Department of Agro Industry, Faculty of Agro Industry and Natural Resources, UMK Campus Jeli, Jeli, Kelantan, Malaysia
Wendy Wee
orcid.org/0000-0002-4399-8937
; Department of Fisheries Science, Faculty of Fisheries and Aqua-Industry, Universiti Malaysia Terengganu, Kuala Terengganu, Terengganu, Malaysia
Zalina Che Manan
; Department of Agro Industry, Faculty of Agro Industry and Natural Resources, UMK Campus Jeli, Jeli, Kelantan, Malaysia
Md Ruhul Amin
; Department of Agro Industry, Faculty of Agro Industry and Natural Resources, UMK Campus Jeli, Jeli, Kelantan, Malaysia
Sukree Hajisamae
; Department of Technology and Industries, Faculty of Science and Technology, Prince of Songkla University, Pattani, Thailand
Abstract
This paper deals with the antibiogram, heavy metal tolerance and genetic properties of Edwardsiella tarda colonizing live Asian Clam, Corbicula fluminea from Pasir Mas, Kelantan, Malaysia. Asian Clam is a popular snack for Malaysians. An antibiotic susceptibility test was carried out using the disc diffusion method against 19 types of antibiotics, namely: oxolinic acid, ampicillin, erythromycin, lincomycin, oleandomycin, amoxicillin, colistin sulphate, sulfamethoxazole, chloramphenicol, doxycycline, florfenicol, flumequine, kanamycin, nalidixic acid, novobiocin, tetracycline, fosfomycin, spiramycin and compound sulphonamides, whereas heavy metal tolerance of bacteria to Hg2+, Cd2+, Cr6+ and Cu2+ was tested using the two fold agar dilution method. The RAPD PCR profile of the bacteria was characterized using three different types of universal primers. It was found that there were most cases of antibiotic sensitivity (56.1%), followed by antibiotic resistance (38.6%) and antibiotic intermediate sensitivity (5.3%). Only kanamycin was found to be able to inhibit all the bacterial isolates present, whereas other bacterial isolates were resistant to fosfomycin, lincomycin, novobiocin and sulphamethoxazole. Additionally, all bacterial isolates were also resistant to all the heavy metals tested. The bacterial isolates present showed a percentage of genetic similarity ranging from 37.5% to 100%. In conclusion, the findings of the present study revealed that the sampled C. fluminea were highly exposed to antibiotic and heavy metal residues.
Keywords
antibiogram; heavy metal; Edwardsiella tarda; Corbicula fluminea
Hrčak ID:
101979
URI
Publication date:
17.5.2013.
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