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Chemical and Biochemical Engineering Quarterly, Vol. 27 No. 2, 2013.

Original scientific paper

Production Optimization,Molecular Characterization and Biological Activities of Exopolysaccharides from Xylaria nigripes

Y.P. Ma ; China Tobacco Henan Industrial Co. Ltd, Zhengzhou, Henan, 450016, P.R.China
D.B. Mao ; College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, 450002, P.R.China
L.J. Geng ; bCollege of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, 450002, P.R.China
W.Y. Zhang ; bCollege of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, 450002, P.R.China
Z. Wang ; bCollege of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, 450002, P.R.China
C.P. Xu ; bCollege of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, 450002, P.R.China

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Abstract

The optimal culture conditions of exopolysaccharides (EPS) production in submerged culture medium by Xylaria nigripes were determined using orthogonal matrix method. The optimal medium (per liter) EPS was 60.0 g L–1 maltose, 1.0 g L–1 peptone,
5 mmol L–1 KH2PO4, and initial pH 7.0 at 28 oC. In the optimal culture medium, the maximum EPS production was 11.967 g L–1 in shake flask. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by size exclusion chromatography
(SEC), and their molecular characteristics were examined by a multiangle laser-light scattering (MALLS) and refractive index (RI) detector system. The weight-average molar masses of Fr-I and Fr-II of EPS were determined to be 6.327104 and 1.478104 g mol–1, respectively. The SEC/MALLS analysis revealed that the molecular
formation of Fr-I is of nearly globular shape. Furthermore, the experiments in vitro indicated that X. nigripes EPS exhibited high antioxidative effects though its antitumour activity was limited.

Keywords

Optimization; exopolysaccharides; Xylaria nigripes; molecular characterization; antioxidant; antitumour

Hrčak ID:

104797

URI

https://hrcak.srce.hr/104797

Publication date:

1.7.2013.

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