Short communication, Note
IMPACT OF OOCYTE SIZE ON LHRHa INDUCEDOVULATION AND FERTILIZED EGG QUALITY IN SADDLED BREAM OBLADA MELANURA (LINNAEUS, 1758)
Nenad Antolović
orcid.org/0000-0002-5360-1542
; University of Dubrovnik, Institute for Marine and Coastal Research, Kneza D. Jude 12, 20000 Dubrovnik, Croatia
Valter Kožul
; University of Dubrovnik, Institute for Marine and Coastal Research, Kneza D. Jude 12, 20000 Dubrovnik, Croatia
Nikša Glavić
; University of Dubrovnik, Institute for Marine and Coastal Research, Kneza D. Jude 12, 20000 Dubrovnik, Croatia
Jakša Bolotin
Abstract
The objective of this study was to evaluate the effects of oocyte size and luteinizing hormone releasing hormone analogue (LHRHa) on ovulatory success in artificial fertilization. Vitellogenic females with maximum oocyte diameters 400-550 µm were repeatedly injected with LHRHa (20 µg kg-1 per injection). Fish with maximum oocyte diameters <500 µm failed to ovulate. In contrast, all fish with maximum oocyte diameters >500 µm spawned within 48-54 h. These results demonstrate that injected LHRHa is effective for ovulation of saddled bream with maximum oocyte diameters >500µm.
Keywords
saddled bream; Oblada melanura LHRH; artificial fertilization
Hrčak ID:
104962
URI
Publication date:
1.7.2013.
Visits: 1.643 *