Original scientific paper

In-vivo assessment of the effects of trypanocidal drugs against Trypanosoma evansi isolates from Philippine water buffaloe (Bubalus bubalis).

Bryan B. Macaraeg ; College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz, Nueva Ecija, Philippines
Jonathan V. Lazaro ; College of Veterinary Science and Medicine, Central Luzon State University, Science City of Munoz, Nueva Ecija, Philippines
Nancy S. Abes ; Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz, Nueva Ecija, Philippines
Claro N. Mingala ; Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz, Nueva Ecija, Philippines

Abstract

The effects of the trypanocidal drugs against Trypanosoma evansi isolated from Philippine water buffaloes from the three island groups were comparatively evaluated. Specifically, the study determined the duration of the efficacy, relapse and death per drug dosage using laboratory mice. A total of 270 inbred Balb/c mice were divided into three groups corresponding to the three trypanosome isolates (Luzon, Visayas, and Mindanao). Each group had three sets, corresponding to the three trypanocidal drugs used with five treatment levels, and one control group each. Each experimental group was composed of five mice. Each mouse was with 0.2 mL of T. evansi intraperitoneally and blood was examined under the microscope. Parasitemia level was determined using the “Rapid Matching Method”. Effective and curative doses were noted and evaluated through t-test and bio-assay graphical analyses. Results showed that the Luzon isolate was sensitive to ≥5mg/kg of diminazene diaceturate and ≥10 mg/kg of both isometamidium chloride and quinapyramine sulphate + chloride. The Visayas isolate was sensitive to ≥5 mg/kg, ≥10 mg/kg, and ≥3 mg/kg of diminazene diaceturate, isometamidium chloride and quinapyramine sulphate + chloride, respectively. The Mindanao isolate was sensitive to ≥3 mg/kg of diminazene diaceturate and quinapyramine sulphate + chloride and 20 mg/kg of isometamidium chloride. The study suggested diminazene as the recommended drug against Luzon isolates, quinapyramine against Visayas isolates and either diminazene or quinapyramine against Mindanao isolates

Keywords

drug sensitivity; trypanocidal drugs; Trypanosoma evansi; Philippines

Hrčak ID:

105379

URI

https://hrcak.srce.hr/105379

Publication date:

8.7.2013.

Article data in other languages: croatian

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