Short communication, Note

Quantitative analysis of polyphenols in eighteen Hypericum taxa

KROATA HAZLER PILEPIĆ ; University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Botany, Schrottova 39, 10000 Zagreb, Croatia
žELJAN MALEŠ ; University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Botany, Schrottova 39, 10000 Zagreb, Croatia

Abstract

Background and Purpose: Phenolics are one of the most ubiquitous
groups of plant secondary metabolites, which play an important role in plant stress tolerance, growth, reproduction, resistance to pathogens, pigmentation and etc. In this study we have analysed the content of polyphenols in the aerial parts of eighteen cultivated Hypericum taxa, collected over two consecutive seasons. No peer-reviewed literature could be found regarding determination of the quantities of polyphenols present in themajority of the analyzed taxa of the native or cultivated samples. Quantitative analysis of phenolic compounds was therefore investigated.

Materials and Methods: The content of phenolic compounds (total
polyphenols, nontannin polyphenols and tannins) in the aerial parts of
eighteenHypericumtaxa collected over two consecutive seasons was determined by spectrophotometric methods.

Results: The quantity of the total polyphenols of investigated taxa (Table 1), ranged from 3.96 to 16.88%. The highest content of total polyphenols was found in the samples of H. kouytchense (16.88%) collected in 2011 andH. hookerianum(14.75%) collected in 2012, while the samples ofH. richeri subsp. grisebachii contained the lowest amount of total polyphenols (4.55 and 3.96%). The average content of tannins in the majority of investigated samples was found to be two times higher than the content of nontannin polyphenols, but in the rest of the samples, themost often ratio of tannins to nontannin polyphenols was 1:1.

Conclusion: The variable content of polyphenols examined here, probably reflects the plant adaptation to seasonal changes in its environment. The occurrence of these metabolites in the taxa explored, hopefully will contribute

Keywords

Hypericum; total polyphenols; nontannin polyphenols; tannins; spectrophotometry; quantitative analysis

Hrčak ID:

110968

URI

https://hrcak.srce.hr/110968

Publication date:

30.9.2013.

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