Original scientific paper

Serum cardiac troponin I as a biomarker in cardiac degeneration following experimental salinomycin toxicosis in sheep.

Ali Hajimohammadi ; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Hamid Rajaian ; Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Elham Khaliji ; Elham Khaliji, Graduate Student, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Saeed Nazifi ; Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Maryam Ansari-Lari ; Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Abstract

Salinomycin is an ionophore with antimicrobial properties. It is a dietary additive used as a growth promoter for ruminants and as a coccidiostat in chickens. However, over-dosage or misuse situations can lead to a series of toxic syndromes. Cardiac troponin I (cTnI) is the part of the troponin complex (I, C and T) within the sarcomere in myocardial cells that regulates contraction of the heart muscle. cTnI is released from injured myocardiocytes into circulation, so it can be a specifific biomarker in myocardial necrosis. The purpose of this study is to propose cTnI for diagnostic cardiac degeneration induced by experimental toxicosis with salinomycin in sheep. Twenty Iranian mixed breed adult female fat-tailed sheep (BW: 33.3 ± 3.4 kg) were used in this study. The sheep were randomly divided in to five equal groups. Group I (control) received 20 mL normal Saline. Groups II, III, IV and V were orally administered 1 mg/kg (twice a day for two days), 2, 3 and 4 mg/kg (once a day for two days) salinomycin, respectively. Following drug administration, blood samples were collected at different time intervals (2, 5, 8, 14 and 21 days) in order to determine various biochemical parameters (cTnI, CK, LDH, ALT and AST). In all groups, the heart sounds of the animals were carefully heard and electrocardiogram (ECG) was taken to determine the type of probable arrhythmia. The results illustrated a significant increase in the activity of cTnI. Numerous arrhythmias were recorded, such as: sinus tachycardia, supraventricular tachycardia, sinus arrhythmia and supraventricular premature contraction. All animals with arrhythmias showed a significant increase in the activity of cTnI. Cardiac muscle necrosis observed macroscopically on post mortem examination revealed myocardial degeneration. Overall, the results of this study indicate that cTnI may be considered as a valuable biomarker in diagnosing cardiac degeneration due to salinomycin toxicosis.

Keywords

salinomycin toxicosis; sheep; troponin I; biochemical parametersM; arrhythmia

Hrčak ID:

116185

URI

https://hrcak.srce.hr/116185

Publication date:

13.2.2014.

Article data in other languages: croatian

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