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Original scientific paper

https://doi.org/10.2478/10004-1254-65-2014-2431

Removal of landfill leachate toxicity and genotoxicity by two treatment methods

Sandra Radić Brkanac ; Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Valerija Vujčić ; Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Petra Cvjetko ; Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Vid Baković ; Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Višnja Oreščanin ; Advanced Energy Ltd., Zagreb, Croatia


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Abstract

Leachates from active and closed municipal solid waste landfills can be a major source of contamination to groundwater and surface waters. In the present study the toxic and genotoxic potential of leachate from an old sanitary landfill prior to and following chemical and electrochemical treatments were assessed using Lemna, Allium, and comet tests. Photosynthetic pigments, malondialdehyde (indicator of lipid peroxidation) and antioxidant enzyme activities were evaluated as additional indicators of toxicity in duckweed. Following duckweed exposure to 25 % dilution of landfill leachate, growth rate and photosynthetic pigments content significantly decreased while lipid peroxidation increased despite stimulation of antioxidative defence mechanisms. Diluted leachate induced DNA strand breaks in duckweed cells as evidenced by the comet assay. Regarding the Allium test, untreated leachate caused inhibition of Allium cepa cell division and induction of mitotic and chromosomal aberrations. Although both water treatments completely reduced genotoxicity of leachate, the electrochemical method was found to be more efficient in removing toxic substances present in landfill leachate and thus more suitable for treating such leachates prior to their discharge into the environment. As landfill leachates pose a risk to human health and environment in general due to their (geno)toxicity, the present study demonstrates that the ecotoxicity/genotoxicity assays should be used in leachate risk assessment together with physicochemical analysis.

Keywords

Allium test; comet assay; Lemna test; oxidative stress indicators; water treatment

Hrčak ID:

117134

URI

https://hrcak.srce.hr/117134

Publication date:

10.3.2014.

Article data in other languages: croatian

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