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Collegium antropologicum, Vol. 37 No. 3, 2013.

Original scientific paper

The Relationship between Methylenetetrahydro-folate Reductase C677T Gene Polymorphism and Diabetic Nephropathy in Croatian Type 2 Diabetic Patients

Nives Gojo Tomić ; University of Zagreb, University Hospital Dubrava, Department of Clinical Pharmacology, Zagreb, Croatia
Srećko Marušić ; University of Zagreb, University Hospital Dubrava, Department of Clinical Pharmacology, Zagreb, Croatia
Velimir Božikov ; University of Zagreb, University Hospital Dubrava, Department of Endocrinology, Zagreb, Croatia
Rajko Kušec ; University of Zagreb, University Hospital Dubrava, Department of Molecular Genetics, Zagreb, Croatia
Vesna Bačić-Vrca ; University of Zagreb, University Hospital Dubrava, Department of Clinical Pharmacy, Zagreb, Croatia
Mario Tadić ; University of Zagreb, University Hospital Dubrava, Department of Gastroenterology, Zagreb, Croatia

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Abstract

Methylenetetrahydrofolate reductase (MTHFR) polymorphism has been shown to be associated with the development of diabetic nephropathy in many ethnic groups. In this study, we examined the correlation between MTHFR C677T poly- morphism and microalbuminuria in patients with diabetes mellitus type 2 in Croatian patients. 85 patients with diabetes mellitus type 2 were recruited. Patients were classified into two groups – with and without diabetic nephropathy ac- cording to urinary albumin excretion rate in urine collected during 24 hours. The C677T genotype was determined by real-time PCR analysis. The genotype frequencies were CC 36,5%, CT 42,3% and TT 21,2% in diabetic patients without nephropathy versus CC 39,4%, CT 45,4% and TT 15,2% in those with nephropathy. There was no statistically significant difference in allele distribution between patients with nephropathy and those without (p=0,788). Our study did not show a correlation between mutations in the MTHFR gene and diabetic nephropathy in Croatian patients. Diabetic nephropathy is influenced by multiple risk factors which can modify the importance of MTHFR polymorphism in its de- velopment.

Keywords

allele distribution; methylenetetrahydrofolate reductase mutations; type 2 diabetes mellitus; diabetic nephropathy; genetic polymorphism; Croatia

Hrčak ID:

118219

URI

https://hrcak.srce.hr/118219

Publication date:

30.9.2013.

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