Veterinary Archives, Vol. 84 No. 2, 2014.
Original scientific paper
The effect of dialysis on the lipid peroxidation and antioxidant status of boar seminal plasma during liquid preservation.
Maja Zakošek Pipan
; Clinic for Reproduction and Horses, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia
Janko Mrkun
; Clinic for Reproduction and Horses, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia
Marjan Kosec
; Clinic for Reproduction and Horses, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia
Alenka Nemec Svete
; Clinic for Small Animal Medicine and Surgery, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia
Petra Zrimšek
; Clinic for Reproduction and Horses, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia
Abstract
The aim of this study was to assess the effect of dialysis on the lipid peroxidation and antioxidant status in liquid stored boar semen. Seventeen ejaculates obtained from eight boars were divided into two aliquots. One aliquot was dialyzed; the other was used as a control. Semen was then stored at 16-17 °C for three days. Sperm quality parameters, capacitation status, thiobarbituric acid reactive substances (TBARS), superoxide dismutase activity (SOD) and total antioxidant capacity (TAC) of dialyzed and non-dialyzed semen were measured. The effect of lipid peroxidation and antioxidant status on semen quality was evaluated. Removal of low molecular weight components (LMWC) from seminal plasma by dialysis resulted in a signifi cantly higher percentage of morphologically normal spermatozoa (P = 0.002). Dialysis moderated the increasing level of acrosomal reacted spermatozoa, showing a significantly lower level of acrosomal reacted spermatozoa in dialyzed semen samples according to each day of storage (P<0.05). SOD increased with storage time in both groups, whereas control samples showed significantly higher levels of SOD than dialyzed ones (P<0.05). Dialyses yielded a significant decrease in TAC and an increase in the level of TBARS (P<0.05). Our results show that elevated levels of SOD in dialyzed semen are not able to counterbalance decreased levels of TAC and prevent oxidative injures to the spermatozoal sperm membrane.
Keywords
semen; porcine; dialysis; antioxidative status; lipid peroxidation
Hrčak ID:
118941
URI
Publication date:
31.3.2014.
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