Short communication, Note

Optimal amplification conditions for D16S3399 polymorphic STS axin-1 gene marker

ANJA KAFKA ; 1_Laboratory of Neurooncology, Croatian Institute for Brain Research, School of Medicine University of Zagreb, Šalata 12, HR-10000 Zagreb, Croatia; 2_Department of Biology, School of Medicine, University of Zagreb, Šalata 3, HR-10000 Zagreb, Croatia
VESNA MUSANI ; Division of Molecular Medicine, Laboratory for Hereditary Cancer, Ruđer Bošković Institute, Bijenička c. 54, HR-10000 Zagreb, Croatia
NIVES PEĆINA-ŠLAUS orcid.org/0000-0002-3334-7671 ; 1_Laboratory of Neurooncology, Croatian Institute for Brain Research, School of Medicine University of Zagreb, Šalata 12, HR-10000 Zagreb, Croatia; 2_Department of Biology, School of Medicine, University of Zagreb, Šalata 3, HR-10000 Zagreb, Croatia

Abstract

We investigated D16S3399 marker and affirmed it as a highly polymorphic
marker useful for the analysis of the human axin-1 gene. Axin-1 acts
as a tumor suppressor gene and its protein is an inhibitor of the Wnt signaling pathway. We report on heterozygosity status, alleles frequency observed in a preliminary group of Croatian subjects and the optimal amplification conditions for D16S3399 marker. The amplified CA repeat was confirmed by direct sequencing.

Keywords

PCR conditions; D16S3399; microsatellite

Hrčak ID:

126366

URI

https://hrcak.srce.hr/126366

Publication date:

31.7.2014.

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