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Archives of Industrial Hygiene and Toxicology, Vol. 65 No. 3, 2014.

Original scientific paper

https://doi.org/10.2478/10004-1254-65-2014-2494

A unique pattern of bisphenol A effects on nerve growth factor gene expression in embryonic mouse hypothalamic cell line N-44

Katsuhiko Warita ; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan
Tomoko Mitsuhashi ; Department of Pathology, University of Pittsburgh, School of Medicine, Pittsburgh, USA
Nobuhiko Hoshi ; Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Kobe, Japan
Ken-ichi Ohta ; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan
Shingo Suzuki ; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan
Yoshiki Takeuchi ; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan
Takanori Miki ; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kagawa, Japan

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Abstract

We investigated the toxicity of bisphenol A (BPA) by determining the gene expression of nerve growth factor (Ngf) in the embryonic mouse cell line mHypoE-N44 derived from the hypothalamus exposed to BPA dose range between 0.02 and 200 μmol L-1 for 3 h. Ngf mRNA levels decreased in a dose-dependent manner, with significant reductions observed in the 2 to 50 μmol L-1 BPA treatment groups compared to controls. However, at 100 to 200 μmol L-1 the Ngf mRNA gradually increased and was significantly higher than control, while the expression of the apoptosis-related genes Caspase 3 and transformation-related protein 73 decreased significantly. These results suggest that in an embryonic hypothalamic cell line the higher doses of BPA induce a unique pattern of Ngf gene expression and that BPA has the potential to suppress apoptosis essential for early-stage brain development.

Keywords

Caspase 3; developmental toxicity; foetal hypothalamus; nerve growth factor; transformation-related protein 73

Hrčak ID:

126612

URI

https://hrcak.srce.hr/126612

Publication date:

11.9.2014.

Article data in other languages: croatian

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