Original scientific paper

P and G genotyping of bovine rotavirus and its prevalence in neonatal calves.

Gantasala V. D. Sravani ; Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
Gurpreet Kaur ; Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
Mudit Chandra ; Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
Padma N. Dwivedi ; Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India

Abstract

To investigate the epidemical characteristics and genotypic distribution of bovine rotavirus in Punjab, 120 fecal samples were collected from calves exhibiting diarrhea and screened for the presence of rotavirus using RNA-PAGE and RT-PCR. Twenty-three samples were positive by RNA-PAGE having electrophoretic patterns that corresponded to mammalian group A rotaviruses. All the samples were (120) screened by RT-PCR for VP7 and VP4 genes and 16 samples were found to be positive for VP7. Out of these 16 samples, 4 samples were also positive for VP4. These were analyzed further by multiplex semi-nested PCR for G and P genotypes, and it was confirmed that 43.75% (7/16) were the G3 type, while 6.25% (1/16) was the G8 type and 50% (8/16) were dual G3G8 types. P genotyping classified all the samples 100% (4/4) as the P[5] genotype. It may be concluded that most prevalent combination of rotavirus infection in bovine was G3G8P[5] in Punjab.

Keywords

bovine rotavirus; RNA-PAGE; RT-PCR; P and G genotyping

Hrčak ID:

128426

URI

https://hrcak.srce.hr/128426

Publication date:

10.10.2014.

Article data in other languages: croatian

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