Original scientific paper

Prevalence and molecular heterogeneity analysis of Campylobacter jejuni and Campylobacter coli isolated from human, poultry and cattle, in Pantnagar, India.

S. Rajagunalan ; Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India
Garima Bisht ; Department of Animal Husbandry, Government of Uttarakhand, Uttarakhand, India
Sheetal Pant ; Department of Animal Husbandry, Government of Uttarakhand, Uttarakhand, India
Suresh P. Singh ; Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Govind Ballabh Pant University of Agriculture and Technology, Uttarakhand, India
Rashmi Singh ; Department of Microbiology, College of Veterinary Sciences and Animal Husbandry, DUVASU, Mathura, Uttar Pradesh, India
Kuldeep Dhama ; Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India

Abstract

Thermophilic campylobacters are the leading cause of food -borne bacterial gastroenteritis worldwide. Reports regarding the prevalence of campylobacters in India are very few and no report on the use of molecular typing tools is available for this important pathogen. In the present study, a total of 612 stool/fecal samples collected from humans (n = 260), poultry (n = 239) and cattle (n = 113) were examined for the presence of thermophilic campylobacters by direct plating on modified charcoal cefoperazone deoxycholate agar plates, and employing conventional morphological and biochemical tests. Of these, only 43 samples showed positive Campylobacter colonies. Further, genus and species level identification and confirmation by multiplex PCR revealed the isolates from human (4) and cattle (1) to be Campylobacter jejuni, whereas, out of 38 isolates from poultry, 29 (76.32%) and 9 (23.68%) were C. coli and C. jejuni, respectively. The genetic diversity of the isolates studied by flaA-RFLP typing, using DdeI restriction enzyme, revealed the presence of 11 and 7 flatypes among the 14 C. jejuni and 29 C. coli isolates, respectively. Dendrogram analysis showed that one of the C. jejuni isolates from poultry shared 100% genetic similarity with the human isolate. The prevalence rate in human, poultry and cattle was estimated to be 1.54, 15.89 and 0.88%, respectively, with a comparatively high prevalence of C. coli in poultry. This study appears to be the first of its kind from India, on the application of multiplex PCR and flaA-RFLP typing of Campylobacter isolates obtained from a variety of sources.

Keywords

Campylobacter; C. jejuni; C. coli; flaA-RFLP typing

Hrčak ID:

128492

URI

https://hrcak.srce.hr/128492

Publication date:

10.10.2014.

Article data in other languages: croatian

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