Original scientific paper

Phylogenetic analysis of canine parvovirus isolates from Mathura, India.

Deepti Singh ; College of Biotechnology, Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishvidhyalaya Evum Go-Anusandhan Sansthan (DUVASU), Mathura, UP, India
Amit K. Verma ; Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Sciences and Animal Husbandry, Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishvidhyalaya Evum Go-Anusandhan Sansthan (DUVASU), Mathura, UP, India
Amit Kumar ; Department of Veterinary Microbiology, College of Veterinary Sciences and Animal Husbandry, Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishvidhyalaya Evum Go-Anusandhan Sansthan (DUVASU), Mathura, UP, India

Abstract

Canine parvovirus (CPV) is an important pathogen causing acute hemorrhagic gastroenteritis in dogs and myocarditis in pups. The present study deals with an analysis of partial nucleotide sequences of the VP1/VP2 gene of CPV isolates from Mathura, India to establish their phylogenetic relationship with other CPV isolates. Out of 100 samples from dogs showing the clinical signs of gastroenteritis viz., vomiting, diarrhea and dysentery, 63 were found positive for CPV-2 by polymerase chain reaction (PCR). Among the 63 positive samples, eight samples were processed further for nucleotide sequencing. Phylogenetic analysis revealed that the CPV variants were not only closely related among themselves but also showed minimum divergence from their ancestors, such as MEV, indicating very little divergence since their origin. From the study, it may be concluded that canine parvovirus-2 variants may represent a potential threat to canine populations. Thus more efforts is required to increase epidemiological monitoring and surveillance, along with the measures necessary
to control this disease in the canine population, and to assess the efficacy of the current vaccines.

Keywords

canine parvovirus; dog; molecular characterization; PCR; phylogenetic analysis

Hrčak ID:

128496

URI

https://hrcak.srce.hr/128496

Publication date:

10.10.2014.

Article data in other languages: croatian

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