Original scientific paper
https://doi.org/10.3325/cmj.2014.55.218
Impact of ovariectomy, high fat diet, and lifestyle modifications on oxidative/antioxidative status in the rat liver
Rosemary Vuković
; Department of Biology, J. J. Strossmayer University of Osijek, Osijek, Croatia
Senka Blažetić
; Department of Biology, J. J. Strossmayer University of Osijek, Osijek, Croatia
Ivana Oršolić
; Department of Biology, J. J. Strossmayer University of Osijek, Osijek, Croatia
Marija Heffer
; Department of Medical Biology, School of Medicine, J. J. Strossmayer University of Osijek Osijek, Croatia
Sandor G. Vari
; International Research and Innovation Management Program Cedars-Sinai Medical Center, Los Angeles, CA, USA
Martin Gajdoš
; Faculty of Medicine, Slovak Medical University in Bratislava, Bratislava, Slovakia
Zora Krivošíková
; Faculty of Medicine, Slovak Medical University in Bratislava, Bratislava, Slovakia
Patrícia Kramárová
; Faculty of Public Health, Slovak Medical University in Bratislava, Bratislava, Slovakia
Anton Kebis
; Faculty of Public Health, Slovak Medical University in Bratislava, Bratislava, Slovakia
Elizabeta Has-Schön
; Department of Biology, J. J. Strossmayer University of Osijek, Osijek, Croatia
Abstract
Aim To estimate the impact of high fat diet and estrogen
deficiency on the oxidative and antioxidative status in the
liver of the ovariectomized rats, as well as the ameliorating
effect of physical activity or consumption of functional
food containing bioactive compounds with antioxidative
properties on oxidative damage in the rat liver.
Methods The study was conducted from November 2012
to April 2013. Liver oxidative damage was determined by
lipid peroxidation levels expressed in terms of thiobarbituric
acid reactive substances (TBARS), while liver antioxidative
status was determined by catalase (CAT), glutathione
peroxidase (GPx), glutathione S-transferase (GST), glutathione
reductase (GR) activities, and glutathione (GSH) content.
Sixty-four female Wistar rats were divided into eight
groups: sham operated and ovariectomized rats that received
either standard diet, high fat diet, or high fat diet
supplemented with cereal selenized onion biscuits or high
fat diet together with introduction of physical exercise of
animals.
Results High fat diet significantly increased TBARS content
in the liver compared to standard diet (P = 0.032, P = 0.030).
Furthermore, high fat diet decreased the activities of CAT,
GR, and GST, as well as the content of GSH (P < 0.050). GPx
activity remained unchanged in all groups. Physical activity
and consumption of cereal selenized onion biscuits
showed protective effect through increased GR activity in
sham operated rats (P = 0.026, P = 0.009), while in ovariectomized
group CAT activity was increased (P = 0.018) in rats
that received cereal selenized onion biscuits.
Conclusion Feeding rats with high fat diet was accompanied
by decreased antioxidative enzyme activities and increased
lipid peroxidation. Bioactive compounds of cereal
selenized onion biscuits showed potential to attenuate the
adverse impact of high fat diet on antioxidative status
Keywords
Hrčak ID:
129837
URI
Publication date:
15.6.2014.
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